Infectious Virions of Are Incorporated into Occlusion Bodies.

The (BmLV) belongs to the unassigned plant virus family and contains a positive-sense, single-stranded RNA genome. BmLV has infected almost all -derived cultured cell lines through unknown routes. The source of BmLV infection and the BmLV life cycle are still unknown.

Expression of recombinant proteins by BEVS in a macula-like virus-free silkworm cell line.

We previously established the first Bombyx mori macula-like virus (BmMLV)-free cell line (BmVF cells) from a B. mori embryo. In this study, we evaluated the expression of recombinant proteins in BmVF cells using a B.

Long-term adaptation of the Bombyx mori BmN4 cell line to grow in serum-free culture.

Bombyx mori ovary-derived BmN4 cells have been successfully adapted to a commercial serum-free medium (SFM; SF900-II) by gradually reducing the serum-containing TC-100 medium content from 100 to 0% (v/v). The BmN4 cells adapted to the SFM (BmN-SFM) adhered strongly to the culture flask and showed altered cell morphology. The BmN-SFM was subcultured 200 times, and the population doubling time was 4.