Variable immersion microscopy with a high numerical aperture.

Three-dimensional (3D) optical microscopy with a high numerical aperture (NA) remains challenging for thick biological specimens owing to aberrations arising from interface refractions. We developed a variable immersion lens (VIL) to passively minimize these aberrations. A VIL is a high-NA concentric meniscus lens and was used in combination with an aberration-corrected high-NA reflecting objective (TORA-FUJI mirror).

Cryogenic Far-Field Fluorescence Nanoscopy: Evaluation with DNA Origami.

Far-field fluorescence localization nanoscopy of individual fluorophores at a temperature of 1.8 K was demonstrated using DNA origami as a one-nanometer-accurate scaffold. Red and near-infrared fluorophores were modified to the scaffold, and the fluorophores were 11 or 77 nm apart.

Nanometer Accuracy in Cryogenic Far-Field Localization Microscopy of Individual Molecules.

We demonstrate the nanometer accuracy of far-field fluorescence localization microscopy at a temperature of 1.8 K using near-infrared and red fluorophores bonded to double-stranded DNA molecules (10.2 nm length).

Prognosis of double crown-retained removable dental prostheses compared with clasp-retained removable dental prostheses: A retrospective study.

Purpose: The present study compared the clinical prognosis of double crown-retained removable dental prostheses (D-DRPs) with that of clasp-retained removable dental prostheses (C-RDPs).

Methods: Clinical records of 201 patients who had received 52 D-RDPs with 144 abutment teeth (D-teeth) and 199 C-RDPs with 399 abutment teeth (C-teeth) at the Prosthetic Dentistry Clinic in Hokkaido University Hospital between April 2005 and June 2015 were analyzed. Survival and complication probabilities of the two types of prostheses and abutment teeth were evaluated using the Kaplan-Meier method and compared using the log-rank test.

Comparison of Treatment Outcomes in Partially Edentulous Patients with Implant-Supported Fixed Prostheses and Removable Partial Dentures.

Purpose: The aim of this study was to compare masticatory performance, occlusal force, and oral health-related quality of life (OHRQoL) in patients with mandibular distal-extension edentulism between those with implant-supported fixed prostheses (ISFPs) and those with removable partial dentures (RPDs), and to evaluate relationships among them.

Materials And Methods: Subjects were recruited from patients using ISFPs or RPDs for mandibular distal-extension edentulism. Masticatory performance was evaluated based on the glucose extracted from chewed gummy jelly.