Multi-attribute method analysis of therapeutic monoclonal antibodies using an automated sample preparation system.

The multi-attribute method (MAM) has attracted increased attention as an alternative strategy for evaluating structural heterogeneity using conventional separation techniques such as ion-exchange chromatography and capillary electrophoresis of therapeutic monoclonal antibodies (mAbs). One of the remaining challenges for the practical use of the MAM is reliable and robust continuous monitoring. In this study, we successfully established an automated sample preparation system as a solution to this issue.