Publications by authors named "Keiko Tsukahara"

We isolated a new strain of the yeast Saccharomyces cerevisiae, 35a14, from banana stems in Okinawa. This strain did not belong to any industrial yeast groups in a phylogenetic tree and produced high levels of alcohol. Furthermore, awamori, an Okinawa's traditional distilled alcoholic beverage, brewed with an l-leucine overproducing mutant derived from 35a14 showed a high concentration of isoamyl acetate.

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Article Synopsis
  • Yeast metabolism is crucial for producing unique flavors in alcoholic beverages like awamori, a traditional spirit from Okinawa, necessitating the optimization of yeast strains for diverse taste profiles.
  • A novel yeast strain, HC02-5-2, isolated from hibiscus flowers, shows high alcohol production and exhibits significant potential for enhancing the flavors in aged awamori due to its ability to produce 4-vinyl guaiacol, a precursor to vanillin.
  • Further breeding of strain HC02-5-2 led to the development of a mutant strain (T25) that accumulates L-leucine, resulting in higher levels of fruity flavors compared to the original strain, showcasing the impact of genetic modification on flavor enhancement
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Background: Cat scratch disease is a common infectious disorder caused by Bartonella henselae that is transmitted primarily by kittens. It typically exhibits a benign and self-limiting course of subacute regional lymphadenopathy and fever lasting two to eight weeks. The most severe complication of cat scratch disease is involvement of the nervous system, such as encephalitis, meningitis, and polyneuritis.

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No standard treatment has been established in childhood blastic plasmacytoid dendritic cell neoplasma (BPDCN) because of its rarity. We treated with acute lymphoblastic leukemia-type regimen for a child with BPDCN with skin and leukemic involvement. She has been disease-free for 4 years after allogeneic bone marrow transplantation in first complete remission.

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