Publications by authors named "Keiichi Minowa"
Post-transcriptional modifications have critical roles in tRNA stability and function. In thermophiles, tRNAs are heavily modified to maintain their thermal stability under extreme growth temperatures. Here we identified 2'-phosphouridine (U) at position 47 of tRNAs from thermophilic archaea.
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- A random mutagenesis method was developed for Thermococcus kodakarensis by using an artificial transposon, aiding in the identification of mutated genes related to thermal sensitivity.
- The study isolated 16 thermosensitive mutants that could not grow at high temperatures (93°C), indicating that their heat tolerance is linked to various genetic functions.
- Mutants showed disruptions in genes responsible for tRNA modifications, leading to lower stability of tRNAs and thus contributing to their temperature sensitivity, highlighting the importance of tRNA modifications for cellular thermotolerance in hyperthermophiles.