[A Case of Gangrenous Cholecystitis Complicated by Ascending Colon Cancer, Successfully Treated with Two-Stage Surgery].

An 80-year-old woman with epigastric pain and weight loss presented to our hospital with cancer of the ascending colon and cholelithiasis. Initially hospitalized for a suspected gallstone attack, she later developed gangrenous cholecystitis. She underwent a laparoscopic cholecystectomy, which revealed abscess formation and necrosis extending into the gallbladder duct.

Higher detectability of gastric cancer after eradication in texture and color enhancement imaging mode 2 in screening endoscopy.

Objectives: The utility of texture and color enhancement imaging (TXI) in detecting gastric cancer (GC) has been investigated. However, few reports exist on TXI mode2 (TXI2) used for detecting GC; this study investigated the efficacy of TXI2 in GC detection during screening endoscopy.

Methods: This study enrolled 13,440 participants with confirmed () infection status who underwent screening endoscopy by 20 endoscopists in our health screening center.

[Malignant lymphoma diagnosed by gallbladder wall thickness in an autoimmune hepatitis patient].

A 77-year-old female patient was undergoing steroid treatment for cirrhosis with autoimmune hepatitis. Periodic imaging acquisitions revealed both irregular gallbladder wall thickness and an isovascular tumor in segment one of the liver. After cholecystectomy and segmental hepatectomy, the pathological diagnosis was diffuse large B-cell lymphoma in both organs.

The rational design of a synthetic polymer nanoparticle that neutralizes a toxic peptide in vivo.

Synthetic polymer nanoparticles (NPs) that bind venomous molecules and neutralize their function in vivo are of significant interest as "plastic antidotes." Recently, procedures to synthesize polymer NPs with affinity for target peptides have been reported. However, the performance of synthetic materials in vivo is a far greater challenge.

Inhibition of Akt (ser473) phosphorylation and rapamycin-resistant cell growth by knockdown of mammalian target of rapamycin with small interfering RNA in vascular endothelial growth factor receptor-1-targeting vector.

Previously we developed dicetyl phosphate-tetraethylenepentamine-based polycation liposomes (TEPA-PCL) for use in small interfering RNA (siRNA) therapy. In the present study, mammalian target of rapamycin (mTOR) expression in cancer cells was silenced with mTOR-siRNA (simTOR) formulated in TEPA-PCL modified with Ala-Pro-Arg-Pro-Gly (APRPG), a peptide having affinity for vascular endothelial growth factor receptor-1 (VEGFR-1). We investigated the effects of inhibition of mTOR, focusing on the differences between cells treated with simTOR and those with rapamycin in terms of Akt (ser473) phosphorylation and antiproliferative effects.

A new method of estimating the cytotoxic effects of suspended particulate matter on cultured alveolar macrophages in vitro.

A new method has been devised for evaluating the cytotoxicity of suspended particulate matters in vitro. It uses the proliferative capacity of mouse alveolar macrophages, estimated by the uptake of (3)H-tymidine, as an index of cytotoxicity, and provides a simple and rapid means of evaluating the cytotoxicity of suspended particulate matter collected on a filter by a personal air sampler. A significant decrease in (3)H-thymidine uptake (an indication of impaired proliferation) could be detected in the range of approximately 0.