Publications by authors named "Kei Kazama"

Objective: To investigate radiographic detection by excretory urography of morphological changes in the urinary bladder associated with urachal anomalies in calves.

Methods: Excretory urography was performed to detect morphological changes in the urinary bladder of 13 calves, of which 6 were nondysuric with swelling of umbilical region and 7 were dysuric without clinical umbilical swelling from November 2022 through April 2024.

Results: The urinary bladder was delineated in all 13 calves after excretory urography.

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  • * Researchers collected nasal swabs from 66 cattle, finding BRBV in 6 symptomatic and 4 asymptomatic animals, and sequenced nearly complete genomes of two Japanese BRBV strains.
  • * The study revealed significant genetic differences in the BRBV leader gene in Japan compared to global strains, highlighting the virus's evolution and genetic diversity influenced by local factors.
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Background And Aim: Mineral deficiencies can lead to dystocia and abnormalities in neonates. Stillbirth of neonatal calves in dairy cows due to dystocia has become an economic problem. Uterine torsion (UT) is a common form of dystocia observed in dairy cows.

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A 28-day-old Holstein calf with astasia (i.e., right hind limb flaccid paralysis) was referred to Large Animal Veterinary Educational Center.

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  • This study evaluated the Charm QUAD2® Test, an immunochromatographic method, for detecting residual macrolides and lincosamides in raw cow's milk, showing high selectivity and a 0% false-positive rate.
  • The test met validation parameters according to European Commission standards, demonstrating low detection limits for various antibiotics that are below most maximum residue limits in Japan, except for lincomycin.
  • Results from the test correlated well with traditional chemical and microbiological methods, indicating its potential for routine food safety analysis in milk.
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The first bovine parechovirus (Bo_ParV) was reported in 2021, and currently, only two nearly complete genome sequences of Bo_ParV are available. In this study, we detected Bo_ParVs in 10 out of 158 bovine fecal samples tested using real-time RT-PCR, and Bo_ParVs were isolated from three of these samples using MA104 cells. Analysis of the P1 region revealed that Bo_ParVs shared high pairwise amino acid sequence similarity (≥ 95.

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Introduction: In sinus floor augmentation, bony nodular prominence at the floor of the maxillary sinus is an obstruction to lateral window approach. It is challenging to detach and elevate the sinus membrane without making any perforations around the nodular prominence, because the membrane is very thin. To overcome these difficulties, we developed a novel method.

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Obesity is one of the risk factors for the onset of various metabolic diseases in dogs. Energy expenditure in brown/beige adipocytes, which is partially regulated by the bone morphogenetic protein (BMP) pathway, is a key factor determining systemic energy balance. Here, we examined gene expression in the fat depots of 129 hospitalized dogs, and the relationship between the relative levels of gene expression and profiles of dogs.

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Parathyroid hormone-related protein (PTHrP) plays essential roles in placental calcium (Ca) transport, and it has been speculated that PTHrP in the placenta is regulated by calcium-sensing receptors (CaSR). This study clarified the relationship between PTHrP in the placenta of dairy cows and minerals in the fetal blood. Blood samples were obtained from 21 Holstein cows and 17 neonatal calves as well as 12 umbilical veins and arteries during cesarean section.

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A 9-month-old Holstein heifer with a history of severely poor growth presented with diarrhoea. On physical examination, a metallic pinging sound was heard on the simultaneous percussion and auscultation of the left trunk. On the cranial abdominal radiography, the contour of a gas-filled balloon-like abomasum wall was delineated, which elevated to the dorsal abdomen.

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Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp.

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