To understand the gut microbiota composition in different intestinal segments is essential to clarify the structure-hypoglycemic relationship of Fructus Mori Polysaccharides (MFP). In this study, the spatial distribution of gut microbiota and fecal metabolites affected by MFP in type 2 diabetes mice was investigated using the 16S rRNA high-throughput sequencing technology and gas chromatography. The results showed that MFP could control body weight and reduce the blood glucose level.
View Article and Find Full Text PDFLactoferrin protein (LF) is a natural protein with certain emulsifying ability, but is sensitive to be affected by environmental factors and has poor oxidative stability to be used as emulsifier. In this study, the emulsifying ability of LF was significantly improved after conjugation with Rosa roxburghii Tratt fruit polysaccharides (RTFP), and the emulsion stability mechanism of LF-RTFP conjugates (L-R) were elucidated through the utilization of CLSM (confocal laser scanning microscopy), interfacial tension, apparent viscosity, and protein adsorption rate. The emulsion stabilized by L-R showed the smaller particle size (17.
View Article and Find Full Text PDFA smartphone-based non-invasive method was developed for salivary uric acid detection using Gleditsia Sinensis carbon dots (GS-CDs). The GS-CDs synthesized by the one-pot hydrothermal method emitted blue fluorescence at a maximum excitation wavelength of 350 nm and had good fluorescence stability in the presence of different ions, while showing selectivity to uric acid solution. The ability of uric acid (UA) to quench the fluorescent substances present in the GS-CDs, was confirmed through HPLC-FLD and LC-MS, FTIR and XPS.
View Article and Find Full Text PDFCinnamon essential oil has gained widespread attention in the food industry as a safe and effective preservative. However, its low water solubility and high volatility limit its application in food, making the use of natural emulsifiers for its emulsification an increasingly popular focus of research. This study focuses on the extraction of galactomannan-rich aqueous extracts from Gleditsia sinensis seeds using a low-energy, low-pollution microwave-assisted method.
View Article and Find Full Text PDFTea polyphenols have been reported to decrease the rate of starch hydrolysis by inhibiting α-glucosidase. However, the effect of the tea harvesting season and the structure of catechin monomers on the inhibitory activity of α-glucosidase is not understood. In this study, the inhibitory effect and underlying mechanism of four seasons of Dancong tea against α-glucosidase were investigated by in vivo and in vitro experiments, multi-spectroscope and molecular dynamic.
View Article and Find Full Text PDFEssential oils have been recognized for their strong antibacterial property, making them an innovative approach for preserving meat. However, their chemical instability and direct impact on meat proteins limit their application. To overcome these limitations, various loading systems have been explored.
View Article and Find Full Text PDFThe study aimed to fabricate β-Lactoglobulin-catechin (β-La-Ca) conjugates as a natural designed antioxidant emulsifier to improve the physicochemical stability of resveratrol emulsion delivery system. Fourier transform infrared (FT-IR) and fluorescence spectroscopy analysis confirmed the formation of conjugates using free radical grafting. The antioxidant ability of emulsion was evaluated by DPPH scavenging activities and ORAC experiments.
View Article and Find Full Text PDFThis study aimed to determine the antibacterial mechanism of cinnamaldehyde fumigation in (). Through vapour fumigation, cinnamaldehyde was confirmed to exhibit effective antibacterial activity against The minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) were 0.25 μL/mL and 0.
View Article and Find Full Text PDFCitronella and Nutmeg are two common spices used for seasoning and medicinal purposes, both of which have significant economic value. This study aimed to investigate whether Citronella essential oil and Nutmeg essential oil (NEO) can ameliorate monosodium urate (MSU)-induced gouty arthritis in rats and the potential mechanisms. The results showed that CEO and NEO reduced swelling and redness at joint sites, inhibited neutrophil infiltration, and limited proinflammatory mediator secretion in mice with MSU-induced gouty arthritis.
View Article and Find Full Text PDFThis study was conducted to investigate the effects of dietary supplementation of 0%, 0.2%, 0.35%, and 0.
View Article and Find Full Text PDFEssential oils (EOs), which are plant-oriented anti-biofilm agents, are extensively encapsulated by cyclodextrins to overcome their aqueous solubility and chemical instability, and achieve slow release during long-term storage. However, the biological activities of EOs decreased after initial encapsulation in CDs. In this study, modified-β-cyclodextrins (β-CDs) were screened as wall materials to maintained the initial anti-biofilm effect of pure CEO.
View Article and Find Full Text PDFIn this study, cinnamon essential oil and tea polyphenols were added to chitosan/ polyvinyl alcohol/ hydroxypropyl methylcellulose/ alizarin composite films to enhance their mechanical and functional properties. Their addition to the composite films enhanced their antibacterial and antioxidant properties and significantly improved its elongation at break (p < 0.05).
View Article and Find Full Text PDFEssential oils (EOs) have good natural antioxidant and antimicrobial properties; however, their volatility, intense aroma, poor aqueous solubility, and chemical instability limit their applications in the food industry. The encapsulation of EOs in -cyclodextrins (-CDs) is a widely accepted strategy for enhancing EO applications. The complexation of cinnamon essential oil (CEO) with five types of -CDs, containing different substituent groups (-CD with primary hydroxyl, Mal--CD with maltosyl, CM--CD with carboxymethyl, HP--CD with hydroxypropyl, and DM--CD with methyl), inclusion process behaviors, volatile components, and antioxidant and antibacterial activities of the solid complexes were studied.
View Article and Find Full Text PDFThe vapor-phase antibacterial activity of essential oils makes them suitable for applications in air disinfection and other fields. At present, vapor-phase antibacterial activity of plant-based essential oils has rarely been reported. Herein, we report a new approach to investigate the antimicrobial activity and mechanism of vapor-phase cinnamaldehyde using () and three other pathogenic bacteria () as model bacteria.
View Article and Find Full Text PDFCompared with traditional liquid-liquid embedding method and solid-liquid embedding method of inclusion complexes of β-cyclodextrin (β-CD) inclusion of essential oil to form stable properties, the gas-liquid embedding method was applied to encapsulate vaporized essential oil (IvEO), with β-CD as wall materials so that core and wall materials molecules are in active state during complexing process. At optimal conditions with a mass ratio of 1:10, temperature of 80°C, time of 1 h, the β-CD-IvEO inclusion complexes (β-CD-IvEO-ICs) had an encapsulation efficiency (EE) of 84.55 ± 2.
View Article and Find Full Text PDFThe emulsifying and antioxidant properties of chicken protein hydrolysates for the physical and oxidative stabilization of chicken oil-in-water emulsion were investigated. The chicken protein pepsin hydrolysates obtained at reaction temperature of 33℃, 1.8% enzyme addition, liquid-solid ratio of 5:1, and reaction time of 4h, showed the DPPH radical scavenging rate of 92.
View Article and Find Full Text PDFThe purpose of this study was to investigate antibacterial activity of essential oil from Cinnamomum camphora var. linaloofera Fujita (EOL) at vapor phase and its mechanism of bactericidal action against . Results showed that the vapor-phase EOL had significant antibacterial activity with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 200 μl/L.
View Article and Find Full Text PDFIn this work, peanut protein isolate (PPI) was grafted with maltodextrin (MD) through the ultrasound-assisted Maillard reaction. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed a link between PPI and MD. The substantially increased accessibility of the major subunits (conarachin, acidic subunit of arachin, and basic subunit of arachin) in PPI under high-intensity ultrasound treatment led to changes in the degree of graft (DG), zeta-potential, protein solubility, and surface hydrophobicity of conjugates.
View Article and Find Full Text PDFA novel flow-injection chemiluminescence (FI-CL) method was described for the determination of sinomenine hydrochloride (SIN). The method was based on the inhibitory effect of SIN on the CL reaction of luminol and K3Fe(CN)6 in an alkaline solution, which was sensitized by CdTe/CdS quantum dots (QDs). Under the optimized conditions, the linear range for the determination of SIN was 1.
View Article and Find Full Text PDFObjective: To know food contamination and genetic diversity of Campylobacter jejuni in four provinces of South China, and to provide data for C. jejuni-associated foodborne disease prevention and control.
Methods: According to the national standard and the most probable number (MPN) method, we detected the contamination of C.
Wei Sheng Wu Xue Bao
February 2003
In this paper, effects of phytohormones on growth and DHA production by Thraustochytrium roseum MF2 were studied. Results showed that phytohormones had significant affect on growth and DHA production by T. roseum MF2.
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