Facilitating bioinformatics reproducibility with QIIME 2 Provenance Replay.

Study reproducibility is essential to corroborate, build on, and learn from the results of scientific research but is notoriously challenging in bioinformatics, which often involves large data sets and complex analytic workflows involving many different tools. Additionally, many biologists are not trained in how to effectively record their bioinformatics analysis steps to ensure reproducibility, so critical information is often missing. Software tools used in bioinformatics can automate provenance tracking of the results they generate, removing most barriers to bioinformatics reproducibility.

Pituitary sellar spine in a pediatric patient with diabetes insipidus.

A sellar spine is a midline bony spur arising from the ventral aspect of the dorsum sellae. Representing a remnant of the anterior notochord, it is often an incidental finding and thought to be of no clinical significance. However, it has recently been suggested that a potential association may exist between sellar spine and pediatric pituitary endocrinopathies, possibly caused by deformation of the developing pituitary gland by the sellar spine.

Safety and efficacy of teriparatide treatment for severe osteoporosis in patients with Duchenne muscular dystrophy.

Unlabelled: Osteoporosis is a major concern in patients with Duchenne muscular dystrophy. In this novel study of teriparatide treatment in 6 patients with severe osteoporosis, bone health (fractures, vertebral morphometry, and DXA) remained stable, with no adverse events. These findings will help inform future osteoporosis research in this challenging population.

Author Correction: Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

ApoE isoform leading to hypertriglyceridemia in new onset type 1 diabetes.

We report a case of pediatric diabetic ketoacidosis (DKA) with significant hypertriglyceridemia. The patient was found to have the e3/ e4 isoform of ApoE, increasing risk of hypertriglyceridemia in DKA. We suggest further genetic investigation for patients presenting with severe hypertriglyceridemia and DKA.

Low-Income Urban Mothers' Experiences with the Supplemental Nutrition Assistance Program.

Background: Food insecurity remains a public health concern in the United States, particularly among low-income urban ethnically diverse families, even with the Supplemental Nutrition Assistance Program (SNAP). Limited phenomenologic data exist to capture how programmatic changes to SNAP address the needs of users and why a gap in SNAP use may exist.

Objective: The primary aim of this study was to examine the experiences of low-income, Baltimore, MD, mothers in applying for and maintaining access to SNAP.

The role of variants regulating metformin transport and action in women with polycystic ovary syndrome.

Aims: Variants in genes encoding metformin transport proteins and the ATM gene are associated with metformin response. We hypothesized that these gene variants contribute to variable metformin treatment response in polycystic ovary syndrome.

Materials & Methods: The discovery cohort (n = 38) was studied in an open-label study.

Canadian student leaders' perspective on interprofessional education: A consensus statement.

The purpose of this article is to report on the outcomes of an interprofessional education (IPE) consensus-building exercise amongst student leaders enrolled in health science-related degree programs. The 12 participants included undergraduate and graduate students from eight different universities situated in five Canadian provinces. Their areas of study spanned a broad range of professions and disciplines including child and youth care, health promotion, nursing, kinesiology, medicine, physical education, psychology, and social work.

Identification of subjects with polycystic ovary syndrome using electronic health records.

Background: Polycystic ovary syndrome (PCOS) is a heterogeneous disorder because of the variable criteria used for diagnosis. Therefore, International Classification of Diseases 9 (ICD-9) codes may not accurately capture the diagnostic criteria necessary for large scale PCOS identification. We hypothesized that use of electronic medical records text and data would more specifically capture PCOS subjects.

Simultaneous measurement of thirteen steroid hormones in women with polycystic ovary syndrome and control women using liquid chromatography-tandem mass spectrometry.

Background: The measurement of adrenal and ovarian androgens in women with PCOS has been difficult based on poor specificity and sensitivity of assays in the female range.

Methods: Women with PCOS (NIH criteria; n = 52) and control subjects with 25-35 day menstrual cycles, no evidence of hyperandrogenism and matched for BMI (n = 42) underwent morning blood sampling. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to simultaneously measure 13 steroids from a single blood sample to measure adrenal and ovarian steroids.

Metformin improves glucose effectiveness, not insulin sensitivity: predicting treatment response in women with polycystic ovary syndrome in an open-label, interventional study.

Context: Although metformin is widely used to improve insulin resistance in women with polycystic ovary syndrome (PCOS), its mechanism of action is complex, with inconsistent effects on insulin sensitivity and variability in treatment response.

Objective: The aim of the study was to delineate the effect of metformin on glucose and insulin parameters, determine additional treatment outcomes, and predict patients with PCOS who will respond to treatment.

Design And Setting: We conducted an open-label, interventional study at an academic medical center.

Cigarette smoking, nicotine levels and increased risk for metabolic syndrome in women with polycystic ovary syndrome.

Women with polycystic ovary syndrome (PCOS) are at risk for metabolic syndrome, which may be exacerbated by smoking. We hypothesized that smoking worsens androgen levels and the metabolic profile in women with PCOS. PCOS smokers (n = 47) and non-smokers (n = 64) and control smokers (n = 30) and non-smokers (n = 28), aged 18-45 years, underwent anthropomorphic measurements, pelvic ultrasound and blood sampling.

The Evaluation and Treatment of Cervical Factor Infertility a Medical-Moral Analysis.

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Reevaluation of the Premier Clostridium difficile toxin A and B immunoassay with comparison to glutamate dehydrogenase common antigen testing evaluating Bartels cytotoxin and Prodesse ProGastro Cd polymerase chain reaction as confirmatory procedures.

Enzyme immunoassays are currently the most common tests used in the clinical laboratory for the detection of Clostridium difficile toxins; however, significant problems with their performance have recently been described. We prospectively reevaluated the Meridian Premier C. difficile toxin A/B assay with direct comparison to a 2-step algorithm that screened for C.

High risk community--men's perceptions of black infant mortality: a qualitative inquiry.

Infant mortality has been identified as a key public health concern in the United States. Although infant mortality rates (IMRs) in the United States have declined during the past 10 years, the rates among Blacks are more than two times higher as compared with other racial and ethnic groups. This study used focus groups to explore Black men's awareness and perceptions of the rising IMR in their community.

A work sampling study of provider activities in school-based health centers.

Background: The purpose of this study was to describe provider activities in a convenience sample of School-Based Health Centers (SBHCs). The goal was to determine the relative proportion of time that clinic staff engaged in various patient care and non-patient care activities.

Methods: All provider staff at 4 urban SBHCs participated in this study; 2 were in elementary schools, 1 in a middle school, and 1 in a school with kindergarten through grade 8.

Improper hydrogen-bonding CH x Y interactions in binary methanol systems as studied by FTIR and Raman spectroscopy.

Fourier Transform infrared spectroscopy and Raman spectroscopy have been used to investigate hydrogen bonding of methanol in different solvents with an aim to explore potential experimental evidence for improper hydrogen bonding involving the methyl group of methanol as suggested by various computational studies. Pure methanol and solutions of methanol in water, acetonitrile, carbon tetrachloride, deuterium oxide, and deuterated acetonitrile have been studied over a range of concentrations. Wavenumber shifts of the CH stretching vibrations were examined to determine if the CH from methanol participates in hydrogen bonding.

Factors related to publication success among faculty development fellowship graduates.

Background And Objectives: This study's purpose was to understand factors related to scholarly project publication among fellowship graduates and to explore the role of collegial relationships and institutional characteristics on ever having published and number of publications.

Methods: We surveyed 5 years of graduates (2000-2004) from the Michigan State University Faculty Development Fellowship Program (n=90) via e-mail. The survey instrument included questions about barriers and facilitators, colleague relationships, and aspects of their institutional environment, the latter adapted from a validated survey by Bland et al.

Infrared optical constants, dielectric constants, molar polarizabilities, transition moments, dipole moment derivatives and Raman spectrum of liquid cyclohexane.

Previous studies have been done in this laboratory focusing on the optical properties of several liquid aromatic and aliphatic hydrocarbons in the infrared. The current study reports the infrared and absorption Raman spectra of liquid cyclohexane. Infrared spectra were recorded at 25 degrees C over a wavenumber range of 7400-490 cm(-1).

Ab initio study of the interaction of CHX3 (X = H, F, Cl, or Br) with benzene and hexafluorobenzene.

In this paper, the results of a study of the interaction of methane, fluoroform, chloroform, and bromoform with benzene and hexafluorobenzene are presented. The benzene complexes were studied at the MP2/6-31G(d) and MP2/6-311++G(2d,p) levels, and the hexafluorobenzene complexes were only studied at the MP2/6-31G(d) level. The optimized geometries, stabilization energies, potential energy surfaces, harmonic frequencies, and vibrational intensities are reported.

Temperature dependence of the optical properties of liquid benzene in the infrared between 25 and 50 degrees C.

The vibrational properties of benzene have long been a topic of interest. A recent comparison presented in the literature of the liquid and gas intensities at 25 degrees C have revealed some intriguing results regarding how the interaction between the hydrogens and the neighbouring pi-clouds in the liquid affect the vibrational intensities. To gain insight into the effect of temperature on the optical properties of liquid benzene and these interactions, the optical constants of liquid benzene have been determined through transmission measurements between 7,400 and 800 cm(-1).