Publications by authors named "Kean-Jin Lim"

The StMADS11 subfamily genes play a crucial role in regulating flowering time, flower development, and bud dormancy in plants. These genes exhibit functional differences between annual and perennial woody plants. In hickory (Carya cathayensis Sarg.

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Phosphatidyl ethanolamine-binding protein (PEBP) plays important roles in plant growth and development. However, few studies have investigated the PEBP gene family in pecan (Carya illinoinensis), particularly the function of the PEBP-like subfamily. In this study, we identified 12 PEBP genes from the pecan genome and classified them into four subfamilies: MFT-like, FT-like, TFL1-like and PEBP-like.

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Article Synopsis
  • Pine wilt disease (PWD) is caused by a tiny worm called the pine wood nematode (PWN), and it harms pine forests around the world.
  • This study looks at how a type of pine tree in China fights against PWN, discovering many genes that change when the tree is infected.
  • The research found that certain chemical pathways in the tree help it defend itself by producing special substances and proteins to fight off the nematode.
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  • Stilbenes play a crucial role in protecting Scots pine heartwood from decay and are induced by various stressors, although specific regulators and enzymes in their synthesis are still unknown.
  • UV-C radiation was identified as the first artificial stressor that activates the stilbene production pathway, prompting a large-scale transcriptomic analysis of pine needles to find potential regulators and missing enzymes.
  • The study highlights similarities in gene regulation between Scots pine and grapevine, suggesting that both ancient and species-specific mechanisms govern stilbene biosynthesis, which could inform breeding programs for more resilient trees.
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The central dogma of genetics, which outlines the flow of genetic information from DNA to RNA to protein, has long been the guiding principle in molecular biology. In fact, more than three-quarters of the RNAs produced by transcription of the plant genome are not translated into proteins, and these RNAs directly serve as non-coding RNAs in the regulation of plant life activities at the molecular level. The breakthroughs in high-throughput transcriptome sequencing technology and the establishment and improvement of non-coding RNA experiments have now led to the discovery and confirmation of the biogenesis, mechanisms, and synergistic effects of non-coding RNAs.

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Pecan (Carya illinoinensis) and Chinese hickory (Carya cathayensis) are important commercially cultivated nut trees. They are phylogenetically closely related plants; however, they exhibit significantly different phenotypes in response to abiotic stress and development. The rhizosphere selects core microorganisms from bulk soil, playing a pivotal role in the plant's resistance to abiotic stress and growth.

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Pecan () nuts are delicious and rich in unsaturated fatty acids, which are beneficial for human health. Their yield is closely related to several factors, such as the ratio of female and male flowers. We sampled and paraffin-sectioned female and male flower buds for one year and determined the stages of initial flower bud differentiation, floral primordium formation, and pistil and stamen primordium formation.

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Pecan () is a popular tree nut. Its fruit development undergoes slow growth, rapid expansion, core hardening, and kernel maturation stages. However, little is known about how pecan initiates fruit development and enlargement after pollination.

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Flower bud differentiation represents a crucial transition from vegetative growth to reproductive development. (hickory) is an important economic species in China, with a long juvenile period that hinders its commercial development. In recent years, circular RNAs (circRNAs) have been widely studied and identified as sponges for miRNA regulation of mRNA expression.

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is a classical and precious dual-use plant for both medicine and food in China. It was first recorded in , and has the traditional functions of nourishing yin, antipyresis, tonifying the stomach, and promoting fluid production. The stem is its medicinal part and is rich in active polysaccharide glucomannan.

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Biomass energy is an essential component of the agriculture economy and represents an important and particularly significant renewable energy source in the fight against fossil fuel depletion and global warming. The recognition that many plants naturally synthesize hydrocarbons makes these oil plants indispensable resources for biomass energy, and the advancement of next-generation sequencing technology in recent years has now made available mountains of data on plants that synthesize oil. We have utilized a combination of bioinformatic protocols to acquire key information from this massive amount of genomic data and to assemble it into an oil plant genomic information repository, built through website technology, including Django, Bootstrap, and echarts, to create the Genomic Information Repository for Oil Plants (GROP) portal (http://grop.

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Hickory, an endemic woody oil and fruit tree species in China, is of great economic value. However, hickory has a long juvenile period and an inconsistent flowering of males and females, thus influencing the bearing rates and further limiting fruits yield. Currently, it is reported that long noncoding RNAs (lncRNAs) play critical regulatory roles in biological processes.

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Due to its peculiar morphological characteristics, there is dispute as to whether the genus of , a species of Juglandaceae, is or . Most morphologists believe it should be distinguished from the genus while genomicists suggest that belongs to the genus. To explore the taxonomic status of using chloroplast genes, we collected chloroplast genomes of 16 plant species and assembled chloroplast genomes of 10 unpublished species.

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We studied the stress response of five-year-old Scots pine xylem to mechanical wounding using RNA sequencing. In general, we observed a bimodal response in pine xylem after wounding. Transcripts associated with water deficit stress, defence, and cell wall modification were induced at the earliest time point of three hours; at the same time, growth-related processes were down-regulated.

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This work investigated the effective doses of biochar (BC) amendment with simulated nitrogen deposition on the stimulation of pecan () growth. A total of nine conditions combining three levels of BC-BC0, 0 t ha year; BC20, 20 t ha year; and BC40, 40 t ha year-and three levels of simulated nitrogen deposition-N0, 0 kg N ha year; N50, 50 kg N ha year; and N150, 150 kg N ha year-were applied throughout 1 year on the pecan-grafted seedlings of cultivar 'Pawnee'. The growth, photosynthesis, chlorophyll and nutrient content in the seedlings were measured.

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Juglandaceae species are plants of great economic value and have been cultivated, domesticated, and utilized by human society for a long time. Their edible, nutrient-rich nuts and tough, durable wood have attracted the attention of botanists and breeders. With the advent of the genomics era, genome sequencing of the Juglandaceae family has been greatly accelerated, and a large amount of data has been generated.

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The SQUAMOSA promoter-binding protein-like (SPL) is a plant-specific transcription factor that influences flowering and vegetative development. Although the SPL genes have been functionally analyzed in many species, studies on the evolutionary history of the whole gene family, and in the Juglandaceae specifically, have been limited. Here, we conducted a phylogenetic relationship analysis of the Juglandaceae SPL gene family compared with other land plant species.

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Article Synopsis
  • Tree bark is a complex structure formed by specialized tissues, mainly developed from two meristems: the phellogen and vascular cambium, which are important for plant protection and growth.
  • The study focused on the silver birch (Betula pendula), analyzing its bark by breaking it down into eight tissue types and using transcriptomics and metabolomics to understand their unique characteristics.
  • Findings highlighted specific metabolic pathways involved in the production of substances like suberin and betulin, and revealed how these pathways have evolved, underscoring the diversity of bark tissues and their potential applications in biotechnology.
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  • The study focused on the role of apoplastic hydrogen peroxide (HO) in lignin metabolism using cell cultures from Norway spruce, highlighting the challenges in studying these processes in whole trees.
  • Scavenging HO hindered lignin formation and revealed that monolignols can be oxidatively coupled in both cell walls and cytoplasm, leading to the production of various phenolic compounds.
  • Transcriptomic analysis showed that reducing apoplastic HO altered gene expression, affecting carbon flow to monolignol production and indicating the apoplast's redox state significantly influences plant metabolism.
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Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make birch an attractive model for forest biotechnology. We assembled and chromosomally anchored the nuclear genome of an inbred B.

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Heartwood extractives are important determinants of the natural durability of pine heartwood. The most important phenolic compounds affecting durability are the stilbenes pinosylvin and its monomethylether, which in addition have important functions as phytoalexins in active defense. A substantial portion of the synthesized pinosylvin is 3-methoxylated but the O-methyltransferase responsible for this modification has not been correctly identified.

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  • Scots pine wood is valued in woodworking for its durability, attributed to heartwood extractives like stilbenes and resin acids.
  • Research investigates whether these extractives are produced in the transition zone between sapwood and heartwood or moved from sapwood.
  • Findings show stilbenes are synthesized in the transition zone using local resources, while resin acids are created in sapwood in spring and transported, with heartwood formation indicated by cell death in summer.
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Identification of distinct allelic versions for dihydroflavonol 4-reductase in gerbera cultivars reveals that gerbera DFR enzymes have strong substrate preference in vivo that is not reflected to the activity in vitro. Flavonoids in the model ornamental plant Gerbera hybrida consist of flavones, flavonols and anthocyanins. Anthocyanins accumulate in the adaxial epidermis of petals and give the different cultivars their characteristic red and violet colour.

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