Three-dimensional tracking of organ development in live plants based on plasma membrane dyes at single-cell resolution.

Plant developmental biology necessitates precise three-dimensional (3D) tracking of dynamic processes in live plants, and the 3D imaging technique in developmental bioimaging requires suitable fluorophores to achieve single-cell resolution imaging. Herein, we have designed a series of plasma membrane fluorescent dyes with a number of excellent properties and established a single-cell resolution imaging tool based on these dyes for three-dimensional imaging of various tissues and organs in living plants. The designed plasma membrane fluorescent dyes not only have the advantages of rapid wash-free staining, highly specific targeting, high brightness and high contrast imaging, ultralong imaging time and low biotoxicity, but also effectively avoid the autofluorescence interference of chlorophyll in cells, allowing for the development of a three-dimensional imaging approach of living plant organs with single-cell resolution.

Machine learning-motivated trace triethylamine identification by bismuth vanadate/tungsten oxide heterostructures.

Triethylamine, an extensively used material in industrial organic synthesis, is hazardous to the human respiratory and nervous systems, but its accurate detection and prediction has been a long-standing challenge. Herein, a machine learning-motivated chemiresistive sensor that can predict ppm-level triethylamine is designed. The zero-dimensional (0D) bismuth vanadate (BiVO) nanoparticles were anchored on the surface of three-dimensional (3D) tungsten oxide (WO) architectures to form hierarchical BiVO/WO heterostructures, which demonstrates remarkable triethylamine-sensing performance such as high response of 21 (4 times higher than pristine WO) at optimal temperature of 190 °C, low detection limit of 57 ppb, long-term stability, reproducibility and good anti-interference property.

Anisotropic hydrogel sensors with muscle-like structures based on high-absorbent alginate fibers.

Hydrogel sensors have attracted much attention as they play a critical role in health monitoring, multifunctional electronic skin, and human-machine interfaces. However, the isotropic structure makes existing hydrogel sensors exhibit isotropic sensing performance. Therefore, it is a challenge to fabricate hydrogels with human tissue-like structures to achieve anisotropic sensing performance.

LAZARUS 1 functions as a positive regulator of plant immunity and systemic acquired resistance.

Systemic acquired resistance (SAR) is activated by local infection and confers enhanced resistance against subsequent pathogen invasion. Salicylic acid (SA) and N-hydroxypipecolic acid (NHP) are two key signaling molecules in SAR and their levels accumulate during SAR activation. Two members of plant-specific Calmodulin-Binding Protein 60 (CBP60) transcription factor family, CBP60g and SARD1, regulate the expression of biosynthetic genes of SA and NHP.

The Dual Resistance Mechanism of CYP325G4 and CYP6AA9 in Legs According to Transcriptome and Proteome Analysis.

Mosquitoes within the complex play a crucial role in human disease transmission. Insecticides, especially pyrethroids, are used to control these vectors. Mosquito legs are the main entry point and barrier for insecticides to gain their neuronal targets.

Feedstock chemical dichloromethane as the C1 source for the chemoselective multicomponent synthesis of valuable 1,4,2-dioxazoles.

The development of mild and practical strategies to produce value-added fine chemicals directly from inexpensive and readily available commodity chemicals is actively pursued by chemists. However, the application of feedstock chemical dichloromethane (DCM) as the C1 source in organic synthesis is still in its infancy. Herein, we describe a multicomponent strategy for the chemoselective synthesis of valuable 1,4,2-dioxazoles by using DCM as a C1 source.

A Study and In Vitro Evaluation of the Bioactive Compounds of Broad Bean Sprouts for the Treatment of Parkinson's Syndrome.

Levodopa (LD) is the first discovered and the most promising and effective medication for Parkinson's disease (PD). As the first identified natural source of LD, L. (broad beans), especially its sprouts, has been confirmed to contain many other potential bioactive compounds that could also be therapeutic for PD.

Sulfated peptides: key players in plant development, growth, and stress responses.

Peptide hormones regulate plant development, growth, and stress responses. Sulfated peptides represent a class of proteins that undergo posttranslational modification by tyrosylprotein sulfotransferase (TPST), followed by specific enzymatic cleavage to generate mature peptides. This process contributes to the formation of various bioactive peptides, including PSKs (PHYTOSULFOKINEs), PSYs (PLANT PEPTIDE CONTAINING SULFATED TYROSINE), CIFs (CASPARIAN STRIP INTEGRITY FACTOR), and RGFs (ROOT MERISTEM GROWTH FACTOR).

Memcapacitors and Memristor Characteristics of ISGE-SOT and SHE-SOT Gain-Driven MoS:Er Ferromagnets.

The enhancement of the spin-orbit torque (SOT) effect through the integration of intrinsic inverse spin galvanic effect spin-orbit torque and spin Hall effect spin-orbit torque is fundamentally dependent on the structural and material properties of the ferromagnets. Consequently, the synthesis of ferromagnets with superior structural integrity and material characteristics is of paramount importance. In this study, a gas-liquid chemical reaction, in conjunction with ultrasonic crushing, was employed to synthesize few-layer MoS:Er nanosheets.

Formulating Spray-Dried Albumin-Modified Lipid Nanoparticles Encapsulating Acyclovir for Enhanced Pulmonary Drug Delivery.

Background: Viral pneumonia, a pressing global health issue, necessitates innovative therapeutic approaches. Acyclovir, a potent ring-opening antiviral agent with broad-spectrum activity, faces water solubility, oral bioavailability, and drug resistance challenges. The aim of this study was to increase the efficacy of acyclovir through respiratory delivery by encapsulating it within albumin-modified lipid nanoparticles and formulate it as a spray.

Tracking Control of Heterogeneous Multiagent Systems With Intrinsic Nonlinear Dynamics in Noisy and Time-Delayed Environments.

This article investigates the tracking control problem of heterogeneous multiagent systems (MASs) with intrinsic nonlinear dynamics in noisy and time-delayed environments. First, a stability criterion for nonlinear stochastic delay systems with multiplicative noise and time-varying delay is proposed by applying the appropriate Lyapunov-Krasovskii functional. Then, based on the proposed stability criterion, sufficient conditions are derived for mean square (m.

Defect-Engineered WO Architectures Coupled with Random Forest Algorithm Enables Real-Time Seafood Quality Assessment.

Reliable and real-time monitoring of seafood decay is attracting growing interest for food safety and human health, while it is still a great challenge to accurately identify the released triethylamine (TEA) from the complex volatilome. Herein, defect-engineered WO architectures are presented to design advanced TEA sensors for seafood quality assessment. Benefiting from abundant oxygen vacancies, the obtained WO sensor exhibits remarkable TEA-sensing performance in terms of higher response (1.

Study on the quality difference between raw and ginger juice processed Magnoliae officinalis cortex by UPLC-Q-TOF-MS/MS and GC-MS coupled with color measurement.

Introduction: Magnoliae officinalis cortex (MOC) has been used for thousands of years as a traditional Chinese herb. In Chinese Pharmacopoeia (2020 edition), it has two types of decoction pieces, raw Magnoliae officinalis cortex (RMOC) and ginger juice processed Magnoliae officinalis cortex (GMOC). The quality difference between RMOC and GMOC has not been explored systemically.

A Bioinspired Membrane with Ultrahigh Li/Na and Li/K Separations Enables Direct Lithium Extraction from Brine.

Membranes with precise Li/Na and Li/K separations are imperative for lithium extraction from brine to address the lithium supply shortage. However, achieving this goal remains a daunting challenge due to the similar valence, chemical properties, and subtle atomic-scale distinctions among these monovalent cations. Herein, inspired by the strict size-sieving effect of biological ion channels, a membrane is presented based on nonporous crystalline materials featuring structurally rigid, dimensionally confined, and long-range ordered ion channels that exclusively permeate naked Li but block Na and K.

Feasibility and safety of ultrasound-guided percutaneous transhepatic measurement of portal venous pressure.

Background And Objective: The measurement of portal venous pressure (PVP) has been extensively studied, primarily through indirect methods. However, the potential of ultrasound-guided percutaneous transhepatic PVP measurement as a direct method has been largely unexplored. This study aimed to investigate the accuracy, safety, and feasibility of this approach.

Salidroside Reduced Ca-CaM-CAMKII-Dependent eNOS/NO Activation to Decrease Endothelial Cell Injury Induced by Cold Combined with Hypoxia.

To investigate vascular endothelium damage in rats exposed to hypoxic and cold and the effect of salidroside in protecting against this damage. A rat isolated aortic ring hypoxia/cold model was established to simulate exposure to hypoxic and cold. The levels of endothelial cell injury markers were measured by ELISA.

Arabidopsis WRKY1 promotes monocarpic senescence by integrative regulation of flowering, leaf senescence, and nitrogen remobilization.

Monocarpic senescence, characterized by whole-plant senescence following a single flowering phase, is widespread in seed plants, particularly in crops, determining seed harvest time and quality. However, how external and internal signals are systemically integrated into monocarpic senescence remains largely unknown. Here, we report that the Arabidopsis thaliana transcription factor WRKY1 plays essential roles in multiple key steps of monocarpic senescence.

Intelligent Gas Detection: g-CN/Polypyrrole Decorated Alginate Paper as Smart Selective NH/NO Sensors at Room Temperature.

Chemiresistive NH/NO sensors are attracting considerable attention for use in air-conditioning systems. However, the existing sensors suffer from cross-sensitivity, detection limit, and power consumption, owing to the inadequate charge-transfer ability of gas-sensing materials. Herein, we develop a flexible NH/NO sensor based on graphitic carbon nitride/polypyrrole decorated alginate paper (AP@g-CN/PPy).

Yak meat content in feed and its impact on the growth of rats.

To evaluate the effects of varying proportions of yak meat in feed on the growth of rats and provide a theoretical basis for selecting the optimal feed proportion suitable for rats. This study was designed as a one-variable experiment. Fifty male rats were divided into five groups.

Charge-regulated fluorescent anchors enable high-fidelity tracking of plasma membrane dynamics during biological events.

Many biological processes generally require long-term visualization tools for time-scale dynamic changes of the plasma membrane, but there is still a lack of design rules for such imaging tools based on small-molecule fluorescent probes. Herein, we revealed the key regulatory roles of charge number and species of fluorescent dyes in the anchoring ability of the plasma membrane and found that the introduction of multi-charged units and appropriate charge species is often required for fluorescent dyes with strong plasma membrane anchoring ability by systematically investigating the structure-function relationship of cyanostyrylpyridium (CSP) dyes with different charge numbers and species and their imaging performance for the plasma membrane. The CSP-DBO dye constructed exhibits strong plasma membrane anchoring ability in staining the plasma membrane of cells, in addition to many other advantages such as excellent biocompatibility and general universality of cell types.

Membralin is required for maize development and defines a branch of the endoplasmic reticulum-associated degradation pathway in plants.

Endoplasmic reticulum (ER)-associated degradation (ERAD) plays key roles in controlling protein levels and quality in eukaryotes. The Ring Finger Protein 185 (RNF185)/membralin ubiquitin ligase complex was recently identified as a branch in mammals and is essential for neuronal function, but its function in plant development is unknown. Here, we report the map-based cloning and characterization of (), which encodes the ER membrane-localized protein membralin and specifically interacts with maize homologs of RNF185 and related components.