Publications by authors named "Ke-yun Song"

Objective: To investigate the risk of H5N1 subtype avian influenza virus (AIV) transmission in the poultry market environment in Changsha city. H5N1 antibody levels among the groups related occupational exposure and AIV nucleic acid in the environment of poultry markets were detected. The characteristics of haemagglutinin (HA) genes of H5N1 AIV in the environment were analyzed.

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Article Synopsis
  • * Nine sewage samples were collected and the NS genes were amplified, sequenced, and analyzed, revealing a 90.1% - 92.6% amino acid identity with reference viruses.
  • * Findings indicated that the NS1 and NS2 proteins exhibited high homology to highly pathogenic strains, suggesting a significant risk for H5N1 virus spread in these environments.
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  • A new RT-LAMP assay was developed for quickly and accurately detecting Norwalk GII, utilizing specially designed primers targeting the RNA-dependent RNA polymerase gene.
  • The assay demonstrated high specificity and sensitivity, successfully identifying 46 out of 48 fecal samples of Norwalk GII without false positives from group A rotaviruses.
  • RT-LAMP was found to have similar detection limits to traditional RT-PCR but offered benefits such as faster results, making it advantageous for outbreak situations involving infectious diarrhea.
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