Puerarin Induces Hepatocellular Carcinoma Cell Apoptosis Modulated by MAPK Signaling Pathways in a Dose-dependent Manner.

Background/aim: Puerarin possesses a battery of therapeutic values in diverse disorders, including pro-apoptotic actions in multiple cancers. Herein, we investigated the effects of puerarin on hepatocellular carcinoma (HCC) in vitro.

Materials And Methods: MTT and flow cytometry were carried out to evaluate the viability and apoptosis of SMMC-7721 HCC cells in the presence of different concentrations of puerarin.

Puerarin inhibits growth and induces apoptosis in SMMC-7721 hepatocellular carcinoma cells.

Puerarin, a predominant isoflavonoid compound extracted from the Chinese medicinal herb Radix Puerariae, is considered to exhibit an antitumor effect. In the present study, the effects of puerarin on SMMC-7721 human hepatocellular carcinoma cells were investigated. Cell viability was assessed by MTT assay.

Clinical characteristics and surgical prognosis of hepatocellular carcinoma with bile duct invasion.

Objectives. Bile duct invasion (BDI) is a rare event in hepatocellular carcinoma (HCC). The present study aimed at investigating clinical characteristics and surgical outcome of HCC patients with bile duct invasion.

[Expression of heparanase and nuclear factor kappa B in pancreatic adenocarcinoma].

Objective: To detect the expressions of heparanase and nuclear factor kappa B p65 (NF-kappaB p65) in pancreatic adenocarcinomas and analyze their relation to patients' prognosis and the regulatory mechanism of NF-kappaB on heparanase expression.

Methods: Heparanase and NF-kappaB p65 proteins in the tumor and adjacent tissues were detected by immunohistochemistry in 48 patients with pancreatic adenocarcinoma and analyzed for their clinicopathological significance.

Results: Heparanase and NF-kappaB p65 proteins were found in 30 (62.

Apoptosis of lymphocytes in allograft in a rat liver transplantation model induced by resveratrol.

Aim: To study the effect of resveratrol (RES) on the apoptosis of lymphocytes in allograft in a rat liver transplantation model.

Methods: Male Sprague-Dawley (SD) rats were selected as donors and male Wistar rats as recipients for a rejection model. The recipients were divided into four groups after orthotopic liver transplantation (OLTx).

Resveratrol prolongs allograft survival after liver transplantation in rats.

Aim: To study the immuno-modulatory effect of resveratrol (RES) on allograft rejection after liver transplantation in rats.

Methods: Male Sprague-Dawley (SD) rats were selected as donors and male Wistar rats as recipients for a rejection model. The recipients were divided into four groups after orthotopic liver transplantation (OLTx).

Rat bone marrow mesenchymal stem cells differentiate into hepatocytes in vitro.

Aim: To investigate the mechanism and regulation of differentiation from bone marrow mesenchymal stem cells (MSCs) into hepatocytes and to find a new source of cell types for therapies of hepatic diseases.

Methods: MSCs were isolated by combining gradient density centrifugation with plastic adherence. The cells were cultured in osteogenic or adipogenic differentiation medium and determined by histochemical staining.

Effects of emodin and double blood supplies on liver regeneration of reduced size graft liver in rat model.

Aim: To study the influences of emodin and reconstruction of double blood supplies on liver regeneration of reduced size graft liver in rat model.

Methods: A total of 45 SD-SD rat reduced size liver transplantation models were randomly divided into three groups (A-C). The conventional reduced size liver transplantation was performed on rats in group A, while the hepatic artery blood supply was restored in groups B and C.

Effect of resveratrol and in combination with 5-FU on murine liver cancer.

Aim: To study the anti-tumor effect of resveratrol and in combination with 5-FU on murine liver cancer.

Methods: Transplantable murine hepatoma22 model was used to evaluate the anti-tumor activity of resveratrol (RES) alone or in combination with 5-FU in vivo. H22 cell cycles were analyzed with flow cytometry.