[An in vitro experiment on the stability and irritant of hypochlorous acid in oral cavity].

Purpose: To study the stability of physicochemical properties and sterilizing effect about two commercially available hypochlorous acid (HClO) products under simulated clinical conditions, and to evaluate the compatibility of HClO on soft and hard tissues and cells in oral cavity.

Methods: Samples of HClO solution with different production processes were prepared, to detect the changes of physicochemical indexes of each sample over time under simulated clinical conditions (shielded from light at 20-25 ℃, open the cover for 5 minutes every day), including free available chlorine, oxidation-reduction potential and pH. Through suspension quantitative germicidal test, the antibiosis-concentration curve of HClO solution was made, so as to calibrate the change of antibacterial ability of disinfectant with the decrease of available chlorine content during storage.

[Intramuscular type of nodular fasciitis: a report of 3 cases and review of literature].

Purpose: To retrospectively analyze the clinical characteristics of impacted supernumerary teeth in 115 patients.

Methods: One hundred and fifteen patients with im-pacted supernumerary teeth who were admitted to the Department of Oral and Max-illofacial Surgery of Hefei Stomatological Hospital were selected randomly. The age, sex, number of teeth, location, direction, clinical manifestation, anaes-thesia method and operation time were analyzed retrospectively, T test and Chi-square test were used to determine the statistical differences with SPSS 19.

[Cytotoxicity of carboxymethyl chitosan zinc and peptide to human periodontal ligament cells].

Purpose: The purpose of this investigation was to study the cytotoxicity of carboxymethyl chitosan zinc and peptide to human periodontal ligament cells in vitro, in order to provide a reference for understanding materials' safety.

Methods: The primary cells were obtained from human periodontal tissues and cultured. The cultured cells were identified by observing the shape under microscope and by immunocytochemical method.

[Expression of OPG/RANK/RANKL in the rat dental pulp tissue of periodontitis combined with vascular calcification and its clinical significance].

Purpose: To study the expression and possible role of OPG/RANK/RANKLin the rat dental pulp of periodontitis combined with vascular calcification.

Methods: Thirty-six male Wister rats were randomly divided into 4 groups: control group(group C), periodontitis group(group CP), vascular calcification group(group VDN) and compound group(group CP+VDN). Each group underwent corresponding management to establish animal model.

[The effect of carboxymethyl chitosan zinc and peptide on IL-1,TNF-α and PGE-2 in gingival crevicular fluid of miniature pigs].

Purpose: This experiment was aimed at exploring whether carboxymethyl chitosan zinc and peptide （CMC-Zn(+)-P） can reduce the occurrence and development of periodontal tissue inflammation effectively by observing the change of IL-1,TNF-α and PGE-2 level in gingival crevicular fluid （GCF） before and after brushing, so as to find a new effective material in preventing and treating periodontal diseases.

Methods: Miniature pigs were selected as experimental subjects and divided into 4 groups randomly: the control group; CMC-Zn(+)-P group (material group);brushing group; brushing + CMC-Zn(+)-P group (composite group). Gingival crevicular fluid before and one month after the experiment was collected.

[Effect of lipopolysaccharide on the expression of ALP,BSP,DSPP in rat dental pulp cells].

Purpose: To study the changes of expression of dentin sialophosphoprotein (DSPP), bone sialoprotein (BSP) and alkaline phosphatase (ALP) in rat dental pulp cells, which were treated with lipopolysaccharide (LPS).

Methods: Rat dental pulp cells were cultured by the method of tissue block in vitro and identified. The cells were treated with Porphyromonas gingivalis (P.

[Expression of core binding factor a1, bone morphogenetic proteins and osteopontin in the developing periodontal tissues of mice].

Objective: To study the expression and interaction of core binding factor a1 (Cbfa1), bone morphogenetic proteins (BMPs) and osteopontin (OPN) in the developing periodontal tissues of mice.

Methods: A mice developing periodontal tissues study model was created histologically by 5-27 day postnatal BALB/c mice, then the immunohistochemical localization of Cbfa1, BMPs and OPN in different developing stages were undertaken.

Results: In early stage of postnatal mice periodontal tissues development, only BMPs expressed in dental follicle cells, though the signal was weak.

[Expression of mineral-associated factors in the mineral stage tooth germ of mice].

Purpose: To study the distribution and expression of core binding factor alpha 1 (Cbf alpha1), bone sialoprotein (BSP), osteopontin (OPN), osteocalcin (OC) and alkaline phosphatase (ALP) in mineral stage tooth germ of mice, so as to understand their roles in the development of the mineral stage tooth germ.

Methods: Thirty BALB/c mice in different days were killed, and their bilateral mandibular first molar germs with surrounding alveolar bone were taken out, then the tissues were fixed with 4% paraformaldehyde at 4 degrees C overnight, dehydrated, embedded in paraffin and serially sectioned at 5 microm. Immunohistochemical assay was adopted to determine the tissue distribution and cellular localization of Cbf alpha1, OPN, BSP, ALP and OC in the mineral stage tooth germ by these sections of BALB/c mice.

[Immunohistochemical localization of leucine-rich proteoglycans in the developing periodontal tissues of mice].

Objective: To study the distribution and expression of fibromodulin, decorin and biglycan in developing normal periodontal tissues, so as to understand its role in periodontal tissue formation.

Methods: Thirty six BALB/c mice in different developing stages were killed and their bilateral mandibular first molars with surrounding alveolar bones and gingival tissues were taken out, Power Vision two steps immunohistochemical method with anti-fibromodulin, anti-decorin and anti-biglycan was used to detect the tissue distribution and cellular localization of fibromodulin and related proteoglycans, decorin and biglycan.

Results: Fibromodulin was strongly expressed in the subcutaneous gingival connective tissue, periodontal ligament, mainly in gingival and periodontal fibroblasts as well as their matrices.

[Expression of Runx2/Cbfa1 in the developing pulpo-dentinal complex of postnatal mice].

Objective: To study the expression of Runx2/Cbfa1 in the developing dentin and differentiating odontoblasts.

Methods: A postnatal mice teeth developing model was built histologically. Immunohistochemical technique was adopted to determine the expression of Runx2/Cbfa1 in the developing pulpo-dentinal complex in mice.

[Histological location of DMP1, OPN and BSP in cementum formation of mouse].

Purpose: To study the distribution of dentin matrix protein 1, osteopontin and bone sialoprotein during cementum formation and cementoblasts differentiation in mouse.

Methods: Thirty six BALB/c postnatal mice were divided into four groups according to the developing stages, nine in each was subdivided into three parts randomly to examine the location of DMP1, OPN and BSP, a tooth developing study model was built and examined using histological methods correspondingly. PV two steps immunohistochemical assay was used to localize the distribution of the three regulatory factors timely and spatially.