Publications by authors named "Ke-ming Xie"
Article Synopsis
- - Oysters, as filter feeders, can accumulate various pathogens, including viruses, which raises concerns for both wild and farmed oysters due to potential diseases but there's limited research on these viruses.
- - The Dataset of Oyster Virome project investigates nearly 30 genomes of oyster-associated CRESS DNA viruses, highlighting their evolutionary diversity and the potential for cross-species transmission.
- - The study reveals a significant number of unclassified CRESS DNA viruses in oyster environments, which enhances our understanding of these viruses' roles and emphasizes the need for further research on their impacts in oysters and ecosystems.
Article Synopsis
- Eighteen new RNA viruses were discovered in a study focused on environmental samples.
- Phylogenetic analysis revealed that these viruses exhibit recombination between key genes, indicating genetic mixing.
- Picobirnaviruses were found to be widespread and plentiful in oyster populations.
RNA viruses have a higher mutation rate than DNA viruses; however, RNA viruses are insufficiently studied outside disease settings. The International Committee on Taxonomy of Viruses (ICTV) is an organization set up by virologists to standardize virus classification. To better understand ICTV taxonomy and the characteristics and rules of different RNA virus families, we analyzed the 3,529 RNA viruses included in the 2020 ICTV report using five widely used metrics: length, host, GC content, number of predicted ORFs, and sequence similarity.
View Article and Find Full Text PDF[Effect of glucosylceramide synthase on P-gp expression by ERK signal transduction pathway in leukemia multi-drug resistance cell line].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
June 2012
Article Synopsis
- The study aimed to explore how glucosylceramide synthase (GCS) influences the expression of P-glycoprotein (P-gp) through ERK signaling pathways in leukemia cells (K562/A02).
- Researchers treated the cells with GCS siRNA and U0126 to analyze the impact on MDR1 mRNA levels and protein expression of P-gp and ERK using qRT-PCR and Western blotting.
- Results showed that inhibiting GCS and using U0126 led to a significant decrease in both P-ERK1/2 and P-gp levels, suggesting that GCS plays a role in modulating P-gp expression via the ERK pathway in leukemia cells.
Previous work from our laboratory demonstrated that glucosylceramide synthase (GCS) and multidrug resistance 1 gene (MDR1) are co-overexpressed in drug-resistant leukemia cells. We hypothesized that GCS and MDR1 may interact. In this study, we used RNA interference (RNAi) to silence the GCS or MDR1 gene in K562/AO2 drug-resistant cells.
View Article and Find Full Text PDF[Study on the correlation of GCS and MDR1 genes in inducing multidrug resistance in human K562/A02 cell line].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
June 2010
Article Synopsis
- The study aims to explore the link between the glucosylceramide synthase (GCS) gene and the multidrug resistance 1 (MDR1) gene in human leukemia cells, specifically K562/A02, to find new ways to overcome drug resistance.
- Researchers used RT-PCR to measure the levels of GCS and MDR1 mRNA in both drug-sensitive and resistant K562 cells, also transfecting specific siRNAs to target these genes.
- Results showed that GCS and MDR1 mRNA levels were significantly higher in drug-resistant K562/A02 cells; silencing GCS led to a notable decrease in MDR1 expression, indicating a positive correlation between these two genes in contributing to drug resistance.
Nometastatic gene 23-H1 (NME1, also known as nm23-H1) is a wide-spectrum tumor metastasis suppressor gene that plays an important role in suppressing the invasion and metastasis of tumor cells. It has been demonstrated that NME1 is also expressed in human first-trimester placenta, but its function at maternal-fetal interface is not clear. The present study aimed to elucidate the biological function of NME1 at the maternal-fetal interface, especially on invasion of the human extravillous cytotrophoblasts (EVCTs).
View Article and Find Full Text PDFGCS induces multidrug resistance by regulating apoptosis-related genes in K562/AO2 cell line.
Cancer Chemother Pharmacol
August 2010
We have previously shown that the expression of glucosylceramide synthase (GCS) gene in drug-resistant K562/AO2 human leukemia cell was higher than that in drug-sensitive K562 cell, and the sensitivity to adriamycin of K562/AO2 cell was enhanced by inhibiting GCS. It is concluded that the overexpression of GCS gene is one of the reasons which lead to multidrug resistance (MDR) of leukemia cell. Meanwhile, we also found that higher expression of Bcl-2 gene and protein were exhibited in K562/AO2 cell compared with K562 cell.
View Article and Find Full Text PDFArticle Synopsis
- Ceramide plays a crucial role in regulating programmed cell death (apoptosis) in leukemia, and Glucosylceramide synthase (GCS) helps cancer cells evade this process by converting ceramide into glucosylceramide.
- A study examined the expression levels of GCS in both clinical leukemia samples and cell lines, finding a strong correlation between high GCS levels and multidrug resistance in leukemia cells.
- The use of a GCS inhibitor, PPMP, demonstrated that blocking GCS increases the sensitivity of resistant leukemia cells to chemotherapy drugs like adriamycin, highlighting the potential of targeting GCS to overcome drug resistance in leukemia treatment.