Effects of Kv1.3 knockout on pyramidal neuron excitability and synaptic plasticity in piriform cortex of mice.

Kv1.3 belongs to the voltage-gated potassium (Kv) channel family, which is widely expressed in the central nervous system and associated with a variety of neuropsychiatric disorders. Kv1.

[Research progress in synthetic biology of active compounds in Chinese medicinal plants].

Mecicinal plants boast abundant natural compounds with significant pharmacological activity, and such compounds, featuring diversified and complex structures, can be used for research and development of drugs. At present, these natural compounds are directly extracted from herbs which, however, suffer from damaged wild resources and shortage of planting resources attributing to the increasing demand. Moreover, the low content in medicinal plants and complex structures are another challenge to the research and development of drugs.

[Studies on the immuno-protection of ROP2 nuclei acid vaccine in Toxoplasma gondii infection].

Objective: To study the protective effect of ROP2 nuclei acid vaccine in mice.

Methods: Forty-two BALB/c mice were divided into three groups. Each mouse in experiment group was injected with 50 microg recombinant plasmid pc-DNA3-ROP2 through musculus quadriceps fexoris.

[Cloning and identification of ROP2 gene of Toxoplasma gondii].

Objective: To amplify ROP2 from the genomic DNA of Toxoplasma gondii RH strain and construct eukaryotic expression plasmid pc-DNA3-ROP2.

Methods: Tachyzoites of T. gondii RH strain were collected and depurated to obtain genome.

Biological role of surface Toxoplasma gondii antigen in development of vaccine.

Aim: To analyze the biological role of the surface antigen of Toxoplasma gondii (T gondii) in development of vaccine.

Methods: The surface antigen of T gondii (SAG1) was expressed in vitro. The immune response of the host to the antigen was investigated by detection of specific antibody reaction to SAG1 and production of cytokines.