Establishment and expression of recombinant human glial cell line-derived neurotrophic factor and TNF α receptor in human neural stem cells.

Objective: To investigate the interference and expression of human glial cell line-derived neurotrophic factor (hGDNF) and soluble TNF alpha (sTNFR I) receptor genes in neural stem cells and to evaluate the roles of these proteins in the genetic treatment of spinal cord injury.

Methods: Full-length of GDNF cDNA (558 bp) and sTNFR I cDNA (504 bp) were inserted into the early 1 region of adenovirus genomic DNA respectively and were immediated by the human cytomegalovirus (gene promoter/enhancer). These adenoviruses were propagated in HEK293 cells via homologous recombination for 7-10 days in vivo, then they were used to infect human neural stem cells.