Publications by authors named "Ke-Nan Ye"
SARS-CoV-2, the culprit pathogen of COVID-19, elicits prominent immune responses and cytokine storms. Intracellular Cl is a crucial regulator of host defense, whereas the role of Cl signaling pathway in modulating pulmonary inflammation associated with SARS-CoV-2 infection remains unclear. By using human respiratory epithelial cell lines, primary cultured human airway epithelial cells, and murine models of viral structural protein stimulation and SARS-CoV-2 direct challenge, we demonstrated that SARS-CoV-2 nucleocapsid (N) protein could interact with Smad3, which downregulated cystic fibrosis transmembrane conductance regulator (CFTR) expression via microRNA-145.
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- - This study investigates the role of intracellular chloride (Cl) in airway epithelial cells and how its accumulation affects airway inflammation, especially after exposure to Pseudomonas aeruginosa lipopolysaccharide (LPS).
- - Researchers found that higher levels of intracellular Cl activated certain signaling pathways (NF-κB and SGK1), leading to increased airway inflammation, while inhibiting SGK1 reduced this inflammation both in lab settings and in animal models.
- - The study highlights the significance of the Cl-SGK1 signaling pathway in conditions like bronchiectasis, and suggests that targeting excessive intracellular Cl levels could be a potential strategy for treating airway inflammatory diseases.
Background: The epithelium lining the epididymis provides an optimal acidic fluid microenvironment in the epididymal tract that enable spermatozoa to complete the maturation process. The present study aims to investigate the functional role of Na(+)/HCO(3)(-) cotransporter in the pH regulation in rat epididymis.
Method/principal Findings: Immunofluorescence staining of pan cytokeratin in the primary culture of rat caput epididymal epithelium showed that the system was a suitable model for investigating the function of epididymal epithelium.