A New Determination Method of the Solubility Parameter of Polymer Based on AIE.

An accurate method of the fluorescence probe approach based on an aggregation-induced emission (AIE) molecule (tetraphenylethylene) for measuring the solubility parameter of the polymer is reported. This method is distinctive in that the approach can make the polymer chain conformation in solution be related to the fluorescence intensity. Since the solubility parameter of the polymer is also closely linked to its chain conformation in solution, the solubility parameter can be determined by the fluorescence intensity.

Determination of the content of residual solvents in muscone by head-space gas chromatography.

Objective: To explore the usefulness of a head-space gas chromatography in the determination of residual solvents including methanol,ethanol,ethyl ether,and petroleum ether in muscone in artificial musk.

Methods: The head-space gas chromatography was performed on HP-5(30 m×0.53 mm,5 Μm)capillary column with a hydrogen flame ionization detector.

Determination of the content of residual solvents in Haikesu 2 by head-space gas chromatography.

Objective: To determine ethylacetate and petroleum ether(60-90 ℃)in Haikesu 2,which is one of the raw materials of artificial musk,using the head-space gas chromatography.

Methods: The determination was performed on HP-5(30 m×0.53 mm,5 Μm)capillary column with an hydrogen flame ionization detector.

Determination of the content of residual solvents in Haikesu I by head-space gas chromatography.

Objective: To explore the usefulness of head-space gas chromatography for the determination of methanol and ethanol in Haikesu I,a raw material of artificial musk.

Methods: The determination was performed on HP-5(30 m×0.53 mm,5 Μm)capillary column with an hydrogen flame ionization detector.

[Study on the global and local calibration methods of tryptophan content in rice by near infrared reflectance spectroscopy].

Tryptophan is one of the essential amino acids in human, and also considered as an important limiting amino acid in rice. Alkali hydrolysis method was used to determine the tryptophan content of 272 representative samples selected from the 1 256 samples in four years. Based on the milled rice flour spectroscopy, by comparing the prediction abilities of the equations with global calibrations and local calibration, the global calibration method with MPLS and the local calibration method were better than others.

Method for umbilical cord blood-derived basophils by FCM.

We have devised a method for the purification of human basophils from umbilical cord blood by FCM. Umbilical cord blood was collected from six healthy, full-term deliveries. After separation of red blood cells, mononuclear cells were isolated by Ficoll-Hypaque.

Protein analysis based on molecular beacon probes and biofunctionalized nanoparticles.

With the completion of the human genome-sequencing project, there has been a resulting change in the focus of studies from genomics to proteomics. By utilizing the inherent advantages of molecular beacon probes and biofunctionalized nanoparticles, a series of novel principles, methods and techniques have been exploited for bioanalytical and biomedical studies. This review mainly discusses the applications of molecular beacon probes and biofunctionalized nanoparticles-based technologies for real-time, , highly sensitive and highly selective protein analysis, including the nonspecific or specific protein detection and separation, protein/DNA interaction studies, cell surface protein recognition, and antigen-antibody binding process-based bacteria assays.

[Ionizing radiation-regulated killing of human hepatoma cells by liposome-mediated CDglyTK gene delivery].

EGR-1 gene promoter and CDglyTK gene were used to construct the pcDNA3-EGR-CDglyTK recombinant vector, in which CDglyTK gene expression was under the control of EGR-1 gene promoter. Cationic liposome LipofectAMINE was used to transfect plasmids into human hepatoma 7402 cell line. Subsequently, the transfected cells were treated with different doses of gamma-ray.

[Combination therapy of experimental murine hepatoma with mIL-12 gene and MHC I gene mediated by liposome].

Background & Objective: Interleukin-12 gene and MHC I gene have been used in gene therapy for cancer individually. To explore the synergistic antitumor effects of these two genes, the therapeutic effects of mIL-12 gene combined with MHC I (mouse H-2K) gene mediated by cationic liposome for experimental murine hepatoma were investigated.

Methods: Balb/C mouse liver cancer MM45T.

A sensitive optode membrane for berberine using conjugated polymer as sensing material.

A new optode membrane for the sensitive determination of berberine based on fluorescence quenching of a conjugated polymer, poly(2,5-dimethoxy-phenyldiacetylene) (PDPA), is proposed. Incorporated in a membrane composed of plasticized poly(vinyl chloride) (PVC), the conjugated polymer exhibits better stability than those small sensing molecules regarding its excellent optical properties and lipophilic characteristics. Moreover, upon the introduction of a negatively charged lipophilic additive (tetraphenylborate salt) into a PVC membrane, the optode displayed enhanced sensitivity.

A selective PVC membrane for di- or trinitrophenol based on N,N-dibenzyl-3,3',5,5'-tetramethylbenzidine.

A new fluorophore, N,N-dibenzyl-3,3',5,5'-tetramethylbenzidine (NBTMB), was prepared and shown to exhibit significant and analytical usefulness for optical sensing toward 2,4-dinitrophenol or 2,4,6-trinitrophenol (picric acid) when it was immobilized in a plasticized poly(vinyl chloride) (PVC) membrane. When the membrane was applied to aqueous nitrophenol solution, NBTMB was able to extract selectively nitrophenol into the membrane phase. Since the extraction equilibrium was accompanied by fluorescence quenching of NBTMB, the chemical recognition process could be directly translated into an optical signal.