The study aims to understand the development of Irritable Bowel Syndrome with Diarrhea (IBS-D) using bioinformatics and differential microRNA analysis in rat colon tissue.
It involves creating an IBS-D model in rats through specific stress techniques, followed by sequencing to identify key microRNAs, particularly miR-6324, and evaluating their target genes and functions through various analytical methods.
The findings suggest that miR-6324 plays a potential role in IBS-D by influencing gene expression related to cell growth and cancer pathways, presenting it as a possible target for future research and treatment strategies.