Publications by authors named "Ke-Geng Wang"

Schistosoma japonicum adults are pre-embedded in a double-layer agar and made the block, then dehydrated with alcohol, isobutyl alcohol and n-butyl alcohol. Various staining procedures can be conducted after conventional sectioning and dewaxing. Complete longitudinal serial sections of the pre-embedded worms can be obtained, and the desired sections can be easily located accurately.

View Article and Find Full Text PDF

Objective: To screen and analyze the peptides in 12 phage-display peptide library specifically binding to the schistosomulum, not cercaria, tegument of Schistosoma japonicum.

Methods: A 12 phage-display peptide library was screened with the S. japonicum schistosomula and cercariae as the target cells for biopanning by degrees, 15 positive clones were picked randomly and deduced by DNA sequencing.

View Article and Find Full Text PDF

The phage titer of samples representing the low, intermediate and high phage number was respectively determined by the double-layer agar plate (DLAP) method and real-time PCR assay. The two methods accurately measured the titer of samples. The plaques from about 1/3 double-agar layer plates could be used to determine the phage titer.

View Article and Find Full Text PDF
Article Synopsis
  • The study aimed to detect Toxoplasma gondii DNA using a technique called loop-mediated isothermal amplification (LAMP).
  • Researchers extracted DNA from T. gondii tachyzoites and designed four primers targeting specific regions of the B1 gene to perform the LAMP assay.
  • Results indicated that LAMP successfully detected T. gondii with high specificity and sensitivity, able to identify as few as 2-3 tachyzoites per reaction, while yielding no positive results from other tested organisms.
View Article and Find Full Text PDF