ACS4 exerts a pivotal role in ethylene biosynthesis during the ripening of tomato fruits in comparison to ACS2.

In the climacteric fruit tomato (Solanum lycopersicum), 1-aminocyclopropane-1-carboxylic acid (ACC) synthase 2 (ACS2) and ACS4 are jointly recognized as key enzymes in orchestrating System-2 ethylene biosynthesis during fruit ripening. However, the precise roles and individual contributions of ACS2 and ACS4 within this process remain elusive. Here, we generate acs2, acs4 single knockout, and acs2/4 double knockout mutants through the CRISPR/Cas9 system.

Utilizing Hybrid Mask and Upsampling Attention Gate for Multiple Immunohistochemistry Image Cell Recognition.

Multi-immunohistochemistry (mIHC) is a crucial technique for simultaneous detection of multiple cellular phenotypes within a single tissue section. Its application in cancer diagnosis and treatment underscores the importance of developing reliable automated cell detection and classification methods for mIHC images. However, existing approaches face significant challenges due to high cell density, heterogeneity, and the laborious nature of annotation.

A stress-driven model for bone density evolution in rats during orthodontic tooth movement.

Orthodontic bone remodeling simulations offer a scientific foundation for optimizing treatment plans and predicting outcomes in orthodontics. Since alveolar bone exhibits unique regional responses and high sensitivity to tensile and compressive stresses, traditional models often fail to account for these characteristics, limiting their accuracy in predicting the microstructural changes of alveolar bone under external forces. To address this issue, this study proposes a bone remodeling model based on equivalent stress derived from the Mohr strength theory as the mechanical stimulus.

A PD-L1 siRNA-Loaded Boron Nanoparticle for Targeted Cancer Radiotherapy and Immunotherapy.

Although the combination of radiotherapy and immunotherapy is regarded as a promising clinical treatment strategy, numerous clinical trials have failed to demonstrate synergistic effects. One of the key reasons is that conventional radiotherapies inevitably damage intratumoral effector immune cells. Boron Neutron Capture Therapy (BNCT) is a precise radiotherapy that selectively kills tumor cells while sparing adjacent normal cells, by utilizing B agents and neutron irradiation.

Temporal multiplexing complex amplitude holography for 3D display with natural depth perception.

In this Letter, we develop a temporal multiplexing complex amplitude holography for a three-dimensional (3D) display with natural depth perception. A series of band-limited random phases is optimized through the designed pseudo incoherent wave propagation model, which can be used as the initialization condition for arbitrary target images. The temporal multiplexing holograms (TMHs) are calculated according to the optical field distribution in the image plane and encoded through the complex amplitude converting method.

Infrared Small Target Detection Algorithm Based on Improved Dense Nested U-Net Network.

Infrared weak and small target detection technology has attracted much attention in recent years and is crucial in the application fields of early warning, monitoring, medical diagnostics, and anti-UAV detection.With the advancement of deep learning, CNN-based methods have achieved promising results in general-purpose target detection due to their powerful modeling capabilities; however, CNN-based methods cannot be directly applied to infrared small targets due to the disappearance of deep targets caused by multiple downsampling operations. Aiming at these problems, we proposed an improved dense nesting and attention infrared small target detection method based on U-Net called IDNA-UNet.

SlMES1 modulates methyl salicylate to influence fruit volatile profiles in tomato.

Methyl salicylate (MeSA), known as phloem-based mobile signal, has been identified as undesirable volatile compounds for tomato fruits due to its medicinal and wintergreen aroma properties. However, the response of most volatile compounds to endogenous MeSA are still unclarified. In this work, we found the concentration of MeSA can be regulated by salicylic acid methyl esterase 1 (SlMES1).

Trajectories of a magnetic sphere in a shaken three-dimensional granular bed under low gravity.

This present investigation employs an advanced magnetic particle tracking method to trace the trajectories of an intruder within a vibration-driven granular medium under artificial low-gravity conditions. The experiments are carried out within the centrifuge of the Chinese Space Station, encompassing six distinct low-gravity environments. Trajectories under various vibration modes are captured and analysed for each gravity level.

Integrating Two Photochromics into One Three-Dimensional Covalent Organic Framework for Synergistically Enhancing Multiple Photocatalytic Oxidations.

While photocatalytic oxidations are a category of important reactions in wide chemical synthesis, the fabrication of effective photocatalysts for broad oxidation reactions is still of a great challenge. Herein, by rationally integrating two photochromics, porphyrin and triphenylamine, a three-dimensional photoactive covalent organic framework (COF) is successfully constructed for photocatalytic oxidations. Characterization studies not only show the formation of a crystalline and three-dimensional porous framework, but also reveal its effective photochemical semiconductor properties derived from the porphyrin and triphenylamine moieties.

Hsp90 and HIF-1α regulate mitophagy by promoting BNIP3 expression in renal ischemia-reperfusion injury.

Background: Ischemia-reperfusion injury (IRI) is a major cause of acute kidney injury (AKI). Studies have shown that mitochondrial damage is involved in the pathogenesis of AKI, and that inhibition of Hsp90 expression can improve IR-induced AKI. However, the mechanisms by which Hsp90 improves IR-induced AKI and whether it is involved in mitochondrial autophagy remain unclear.

Dual oxygen supply system of carbon dot-loaded microbubbles with acoustic cavitation for enhanced sonodynamic therapy in diabetic wound healing.

Diabetic wounds present significant treatment challenges due to their complex microenvironment, marked by persistent inflammation from bacterial infections, hypoxia caused by diabetic microangiopathy, and biofilm colonization. Sonodynamic therapy (SDT) offers potential for treating such wounds by targeting deep tissues with antibacterial effects, but its efficacy is limited by hypoxic conditions and biofilm barriers. To overcome these obstacles, we developed a novel approach using oxygen-carrying microbubbles loaded with Mn-doped carbon dots (MnCDs@OMBs) to enhance SDT and disrupt biofilms.

Phenylboronic acid-modified carbon dot-proteinase K nanohybrids for enhanced photodynamic therapy against bacterial biofilm infections.

Nanohybrids combining phenylboronic acid-modified carbon dots (PCDs) and proteinase K have been engineered for addressing the formidable challenges of antimicrobial photodynamic therapy (aPDT) against bacterial biofilm infections, overcoming biofilm barrier obstruction, the limited diffusion of reactive oxygen species (ROS), and the inadequate ROS generation of traditional photosensitizers. PCDs are formulated for superior water solubility and robust singlet oxygen (O) production, mitigating issues related to dispersion and aggregation-induced quenching typical of conventional photosensitizers. The conjugation of phenylboronic acid to CDs not only enhanced O generation through increased electron-hole separation but also imparted strong bacterial binding capabilities to the PCDs, enabling broad-spectrum sterilization by maximizing the ROS-mediated bacterial destruction.

A new effLuc/Kate dual reporter allele for tumor imaging in mice.

Genetically engineered mouse models (GEMMs) are instrumental for modelling local and systemic features of complex diseases, such as cancer. Non-invasive, longitudinal cell detection and monitoring in tumors, metastases and/or the micro-environment is paramount to achieve a better spatiotemporal understanding of cancer progression and to evaluate therapies in preclinical studies. Bioluminescent and fluorescent reporters marking tumor cells or their microenvironment are valuable for non-invasive cell detection and monitoring in vivo.

Durable and deep response to CVD chemotherapy in SDHB-mutated metastatic paraganglioma: case report.

Introduction: Succinate dehydrogenase subunit B (SDHB)-mutated paragangliomas (PGLs) are rare neuroendocrine tumors characterized by increased malignancy, readily metastasizing, and poorer prognosis. Here we report a case of SDHB-mutated metastatic PGL, wherein the patient showed significant tumor shrinkage and complete symptom remission following chemotherapy. We aim to contribute additional evidence to the existing knowledge associated with SDHB-mutated PGLs.

Considerations and Indications for Gastric Emptying Scintigraphy in Lung Transplant Patients.

Objectives: Gastroparesis is a complication following lung transplantation. This study aimed to assess the prevalence of gastroparesis in patients with lung transplants undergoing solid phase gastric emptying scintigraphy (GES). Specifically, we investigated which type of lung transplant is more susceptible to gastroparesis and whether timing of GES post-transplantation impacts diagnosis of severe gastroparesis.

Efficacy and safety of S-1 plus oxaliplatin combined with apatinib and camrelizumab as neoadjuvant therapy for patients with locally advanced gastric or gastroesophageal junction adenocarcinoma: a protocol for a single-arm phase II trial.

Gastric cancer, as the fifth most diagnosed malignancy and the fourth leading cause of cancer-related death globally, remains a significant health concern. The potential effect of the programmed death-1 (PD-1) inhibitor, when used alongside chemotherapy and antiangiogenic agents in neoadjuvant therapy for gastric cancer, has yet to be explored in the published literature. This study aims to evaluate the efficacy and safety of the S-1 plus oxaliplatin (SOX) regimen when combined with apatinib and camrelizumab (SOXAC) as neoadjuvant therapy for patients with locally advanced gastric or gastroesophageal junction (GEJ) adenocarcinoma.

Lipidomic analysis of plant-derived extracellular vesicles for guidance of potential anti-cancer therapy.

Plant-derived extracellular vesicles (PEVs) have been regarded as a superior source for nanomedicine and drug delivery systems. Nevertheless, their clinical translation is hindered by the lack of clarity and even contradiction in their biomedical applications. Herein, we conducted a comprehensive compositional analysis of four commonly used PEVs to fully understand their functional lipid contents and assess their potential therapeutic applications.

Hippocampus carbonic anhydrase 1 via ERK pathway may be involved in depressive like behaviors.

Major depressive disorder (MDD) is a destructive mental disease, yet the mechanism is still not clear. Carbonic anhydrase, an efficient catalyst for CO conversion to carbonate and protons, could affect many functions, such as memory formation recognition. Lately, we illustrated that carbonic anhydrase 1 (CAR1) knockout (CAR1) mice could lead to depressive-like behaviors, but the underlying molecular mechanism is unknown.

Progress and Gaps in Respiratory Disease Research and Treatment: Highlights of the IRM 2024 in Shanghai.

Respiratory diseases pose a major public health challenge globally, necessitating collaborative efforts between basic researchers and clinicians for effective solutions. China, which is heavily impacted by a broad spectrum of respiratory disorders, has made notable strides in both research and clinical management of these diseases. The International Respiratory Medicine (IRM) meeting was organized with the primary goal of facilitating the exchange of recent research developments and promoting collaboration between Chinese and American scientists in both basic and clinical research fields.

Zinc cadmium sulphide-based photoreforming of biomass-based monosaccharides to lactic acid and efficient hydrogen production.

Approaches that add value to biomass through the use of photoreforming reactions offer great opportunities for the efficient use of renewable resources. Here, we constructed a novel zinc cadmium sulphide/molybdenum dioxide-molybdenum carbide-carbon (ZnCdS-y/MoO-MoC-C) heterojunction which was applied to photoreforming of biomass-based monosaccharides for hydrogen and lactic acid production. Bandgap engineering effectively modulated the redox capacity of ZnCdS-y and exposed more (101) crystalline surfaces, which improved the lactic acid selectivity.

Combination of triple chemotherapy and sequential re-irradiation as salvage for recurrent treatment-refractory hemangiopericytoma of extraspinal dura: a case report.

Introduction: Hemangiopericytoma (HPC) in the central nervous system (CNS) is rare. Our report aims to present an HPC case with multiple surgeries at the lumbar spine, and demonstrates an effective treatment as salvage.

Case Report: In this report, we present the case of a young girl with recurrent meningeal invasion of lumbar spinal HPC.

Extracellular vesicle therapeutics for cardiac repair.

Extracellular vesicles (EVs) are cell-secreted heterogeneous vesicles that play crucial roles in intercellular communication and disease pathogenesis. Due to their non-tumorigenicity, low immunogenicity, and therapeutic potential, EVs are increasingly used in cardiac repair as cell-free therapy. There exist multiple steps for the design of EV therapies, and each step offers many choices to tune EV properties.