Publications by authors named "Kazuyasu Miyoshi"
Background: The cross-reactive antibody response against seasonal human coronaviruses (HCoVs) was evaluated according to disease severity in patients with COVID-19 in Japan.
Methods: In total, 194 paired serum samples collected from 97 patients with COVID-19 (mild, 35; severe, 62) were analyzed on admission and during convalescence. IgG antibodies against the nucleocapsid (N) and spike (S) proteins of SARS-CoV-2 and four seasonal HCoVs (HCoV-NL63, -229E, -OC43, and -HKU1) were detected by enzyme-linked immunosorbent assays.
Article Synopsis
- Serological tests for COVID-19 play a vital role in diagnosis and surveillance, yet there are limited studies exploring their clinical utility and antibody response in relation to disease severity.* -
- The study evaluated the VITROS SARS-CoV-2 antibody tests on serum samples from 130 patients in Japan, categorizing them into mild and severe COVID-19 cases to assess the relationship between antibody presence and clinical outcomes.* -
- It was found that while antibody levels increased with severity after 11 days from symptom onset, clinical history and lab results at admission were better predictors of disease prognosis, highlighting the importance of a comprehensive diagnostic approach.*
Article Synopsis
- * Higher levels of IgM and IgG antibodies against the nucleocapsid (N) and spike 1 (S1) proteins were observed in patients with severe and critical cases compared to mild cases, particularly noticeable around 11 days after symptom onset.
- * The research indicated that severe and critical cases experienced a stronger antibody response, with higher seroconversion rates of IgG and IgM, while mild cases showed significantly lower rates of antibody development.
The causative agent of the COVID-19 pandemic, SARS-CoV-2, is steadily mutating during continuous transmission among humans. Such mutations can occur in the spike (S) protein that binds to the ACE2 receptor and is cleaved by TMPRSS2. However, whether S mutations affect SARS-CoV-2 cell entry remains unknown.
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- - The study evaluated the effectiveness of six molecular diagnostic tests and a rapid antigen test for detecting COVID-19 in self-collected saliva from 103 patients, including both symptomatic and asymptomatic cases.
- - Molecular tests detected SARS-CoV-2 RNA in 50.5% to 81.6% of saliva samples, with higher detection rates (65.6% to 93.4%) within 9 days of symptom onset, while the rapid antigen test only identified the virus in 11.7% of samples.
- - The findings suggest that self-collected saliva is a viable option for COVID-19 diagnosis, with several molecular tests showing sufficient accuracy, whereas the rapid antigen test is not recommended for initial diagnosis
Article Synopsis
- The study investigates the clinical features of COVID-19 among passengers of the Diamond Princess cruise ship to identify markers for symptom onset and disease progression.
- It involved a retrospective analysis of 104 patients who were admitted to a hospital in Tokyo with confirmed SARS-CoV-2 infections between February 11 and February 25, 2020.
- Key findings showed that, at the end of the observation period, 32% remained asymptomatic, 41% had mild symptoms, and 27% developed severe COVID-19, with factors like serum lactate hydrogenase levels taking precedence in identifying symptom severity.
Background: We evaluated the clinical performance of an immunochromatographic (IC) IgM/IgG antibody assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) and chest computed tomography (CT) for the diagnosis of Coronavirus disease 2019 (COVID-19).
Methods: We examined 139 serum specimens collected from 112 patients with COVID-19 and 48 serum specimens collected from 48 non-COVID-19 patients. The presence of IgM/IgG antibody for SARS-COV2 was determined using the One Step Novel Coronavirus (COVID-19) IgM/IgG Antibody Test.
Legionella pneumophila is recognized as a common causative organism for community-acquired pneumonia, but it is rarely a causative organism for hospital-acquired pneumonia, except in cases of hospital outbreak. Recently, most of the Legionella cases have been diagnosed using the urine antigen test. However, this test can reliably detect only L.
View Article and Find Full Text PDF[The Great Imitator; Clavicular Tuberculosis Mimics a Metastatic Neoplasm].
Kansenshogaku Zasshi
September 2015
In the same manner as syphilis, tuberculosis (TB) was often called "The Great Imitator". We have to consider not only malignancies but also TB as a differential diagnosis when we find any tumorous regions. We report herein on a rare case, clavicular osteomyelitis due to TB.
View Article and Find Full Text PDFIn 2014, we reported the first trial based on outpatient parenteral antimicrobial therapy (OPAT) with continuous infusions in Japan. Following this, we found many patients who were eligible for OPAT but could not undertake it owing to difficulties in accessing the clinic daily. To overcome this problem, we created a model in collaboration with visiting nursing stations and started providing OPAT with the services.
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