Extreme deformability of insect cell membranes is governed by phospholipid scrambling.

Organization of dynamic cellular structure is crucial for a variety of cellular functions. In this study, we report that Drosophila and Aedes have highly elastic cell membranes with extremely low membrane tension and high resistance to mechanical stress. In contrast to other eukaryotic cells, phospholipids are symmetrically distributed between the bilayer leaflets of the insect plasma membrane, where phospholipid scramblase (XKR) that disrupts the lipid asymmetry is constitutively active.

Particle Timing Control and Alignment in Microchannel Flow by Applying Periodic Force Control Using Dielectrophoretic Force.

In this study, a technique for particle streamwise timing, spacing and velocity control (alignment) in microchannel flow by controlling the forces exerted on the particle in space and time, was developed. In the present technique, the timing of particles crossing a certain position in microchannel flow with a specific interval and the particle velocity are controlled by applying acceleration and deceleration forces periodically in the streamwise direction and activating them periodically. The force is produced by a dielectrophoretic force using ladder-type electrodes embedded in the microfluidic device and is turned on and off in a cycle.

Single-cell cloning and expansion of human induced pluripotent stem cells by a microfluidic culture device.

The microenvironment of cells, which includes basement proteins, shear stress, and extracellular stimuli, should be taken into consideration when examining physiological cell behavior. Although microfluidic devices allow cellular responses to be analyzed with ease at the single-cell level, few have been designed to recover cells. We herein demonstrated that a newly developed microfluidic device helped to improve culture conditions and establish a clonality-validated human pluripotent stem cell line after tracing its growth at the single-cell level.

Numerical and experimental study on the development of electric sensor as for measurement of red blood cell deformability in microchannels.

A microsensor that can continuously measure the deformability of a single red blood cell (RBC) in its microchannels using microelectrodes is described in this paper. The time series of the electric resistance is measured using an AC current vs. voltage method as the RBC passes between counter-electrode-type micro-membrane sensors attached to the bottom wall of the microchannel.