Efficient proximal tubule-on-chip model from hiPSC-derived kidney organoids for functional analysis of renal transporters.

Renal transporters play critical roles in predicting potential drug-drug interactions. However, current models often fail to adequately express these transporters, particularly solute carrier proteins, including organic anion transporters (OAT1/3), and organic cation transporter 2 (OCT2). Here, we developed a hiPSC-derived kidney organoids-based proximal tubule-on-chip (OPTC) model that emulates renal physiology to assess transporter function.

Random Matrix Statistics in Propagating Correlation Fronts of Fermions.

We theoretically study propagating correlation fronts in noninteracting fermions on a one-dimensional lattice starting from an alternating state, where the fermions occupy every other site. We find that, in the long-time asymptotic regime, all the moments of dynamical fluctuations around the correlation fronts are described by the universal correlation functions of Gaussian orthogonal and symplectic random matrices at the soft edge. Our finding here sheds light on a hitherto unknown connection between random matrix theory and correlation propagation in quantum dynamics.

Deciphering potential vascularization factors of on-chip co-cultured hiPSC-derived cerebral organoids.

The lack of functional vascular system in stem cell-derived cerebral organoids (COs) limits their utility in modeling developmental processes and disease pathologies. Unlike other organs, brain vascularization is poorly understood, which makes it particularly difficult to mimic . Although several attempts have been made to vascularize COs, complete vascularization leading to functional capillary network development has only been achieved transplantation into a mouse brain.

SARS-CoV-2-induced disruption of a vascular bed in a microphysiological system caused by type-I interferon from bronchial organoids.

Blood vessels show various COVID-19-related conditions including thrombosis and cytokine propagation. Existing blood vessel models cannot represent the consequent changes in the vascular structure or determine the initial infection site, making it difficult to evaluate how epithelial and endothelial tissues are damaged. Here, we developed a microphysiological system (MPS) that co-culture the bronchial organoids and the vascular bed to analyze infection site and interactions.

Pericytes and shear stress each alter the shape of a self-assembled vascular network.

Blood vessel morphology is dictated by mechanical and biochemical cues. Flow-induced shear stress and pericytes both play important roles, and they have previously been studied using on-chip vascular networks to uncover their connection to angiogenic sprouting and network stabilization. However, it is unknown which shear stress values promote angiogenesis, how pericytes are directed to sprouts, and how shear stress and pericytes affect the overall vessel morphology.

Long-term effect of sodium selenite on the integrity and permeability of on-chip microvasculature.

Development of the robust and functionally stable three-dimensional (3D) microvasculature remains challenging. One often-overlooked factor is the presence of potential anti-angiogenic agents in culture media. Sodium selenite, an antioxidant commonly used in serum-free media, demonstrates strong anti-angiogenic properties and has been proposed as an anticancer drug.

Impact of Dissipation on Universal Fluctuation Dynamics in Open Quantum Systems.

Recent theoretical and experimental works have explored universal dynamics related to surface growth physics in isolated quantum systems. In this Letter, we theoretically elucidate that dissipation drastically alters universal particle-number-fluctuation dynamics associated with surface-roughness growth in one-dimensional free fermions and bosons. In a system under dephasing that causes loss of spatial coherence, we numerically find that a universality class of surface-roughness dynamics changes from the ballistic class to a class with the Edwards-Wilkinson scaling exponents and an unconventional scaling function.

Durability of Aligned Microtubules Dependent on Persistence Length Determines Phase Transition and Pattern Formation in Collective Motion.

Collective motion is a ubiquitous phenomenon in nature. The collective motion of cytoskeleton filaments results mainly from dynamic collisions and alignments; however, the detailed mechanism of pattern formation still needs to be clarified. In particular, the influence of persistence length, which is a measure of filament flexibility, on collective motion is still unclear and lacks experimental verifications although it is likely to directly affect the orientational flexibility of filaments.

Linear-Zero Mode Waveguides for Single-Molecule Fluorescence Observation of Nucleotides in Kinesin-Microtubule Motility Assay.

Single-molecule fluorescence microscopy is a key tool to investigate the chemo-mechanical coupling of microtubule-associated motor proteins, such as kinesin. However, a major limitation of the implementation of single-molecule observation is the concentration of fluorescently labeled molecules. For example, in total internal reflection fluorescence microscopy, the available concentration is of the order of 10 nM.

Three-dimensional tissue model in direct contact with an on-chip vascular bed enabled by removable membranes.

Three-dimensional (3D) tissue culture is a powerful tool for understanding physiological events. However, 3D tissues still have limitations in their size, culture period, and maturity, which are caused by the lack of nutrients and oxygen supply through the vasculature. Here, we propose a new method for culturing a 3D tissue-a spheroid-directly on an 'on-chip vascular bed'.

Dynamical Scaling of Surface Roughness and Entanglement Entropy in Disordered Fermion Models.

Localization is one of the most fundamental interference phenomena caused by randomness, and its universal aspects have been extensively explored from the perspective of one-parameter scaling mainly for static properties. We numerically study dynamics of fermions on disordered one-dimensional potentials exhibiting localization and find dynamical one-parameter scaling for surface roughness, which represents particle-number fluctuations at a given length scale, and for entanglement entropy when the system is in delocalized phases. This dynamical scaling corresponds to the Family-Vicsek scaling originally developed in classical surface growth, and the associated scaling exponents depend on the type of disorder.

Growth rate-dependent flexural rigidity of microtubules influences pattern formation in collective motion.

Background: Microtubules (MTs) are highly dynamic tubular cytoskeleton filaments that are essential for cellular morphology and intracellular transport. In vivo, the flexural rigidity of MTs can be dynamically regulated depending on their intracellular function. In the in vitro reconstructed MT-motor system, flexural rigidity affects MT gliding behaviors and trajectories.

Magnetic Solitons in a Spin-1 Bose-Einstein Condensate.

Vector solitons are a type of solitary or nonspreading wave packet occurring in a nonlinear medium composed of multiple components. As such, a variety of synthetic systems can be constructed to explore their properties, from nonlinear optics to ultracold atoms, and even in metamaterials. Bose-Einstein condensates have a rich panoply of internal hyperfine levels, or spin components, which make them a unique platform for exploring these solitary waves.

Family-Vicsek Scaling of Roughness Growth in a Strongly Interacting Bose Gas.

Family-Vicsek scaling is one of the most essential scale-invariant laws emerging in surface-roughness growth of classical systems. In this Letter, we theoretically elucidate the emergence of the Family-Vicsek scaling even in a strongly interacting quantum bosonic system by introducing a surface-height operator. This operator is comprised of a summation of local particle-number operators at a simultaneous time, and thus the observation of the surface roughness in the quantum many-body system and its scaling behavior are accessible to current experiments of ultracold atoms.

Synthetic dissipation and cascade fluxes in a turbulent quantum gas.

Scale-invariant fluxes are the defining property of turbulent cascades, but their direct measurement is a challenging experimental problem. Here we perform such a measurement for a direct energy cascade in a turbulent quantum gas. Using a time-periodic force, we inject energy at a large length scale and generate a cascade in a uniformly trapped three-dimensional Bose gas.

Flemish Strings of Magnetic Solitons and a Nonthermal Fixed Point in a One-Dimensional Antiferromagnetic Spin-1 Bose Gas.

Thermalization in a quenched one-dimensional antiferromagnetic spin-1 Bose gas is shown to proceed via a nonthermal fixed point through annihilation of Flemish-string bound states of magnetic solitons. A possible experimental situation is discussed.

Simultaneous Observation of Kinesin-Driven Microtubule Motility and Binding of Adenosine Triphosphate Using Linear Zero-Mode Waveguides.

Single-molecule fluorescence observation of adenosine triphosphate (ATP) is a powerful tool to elucidate the chemomechanical coupling of ATP with a motor protein. However, in total internal reflection fluorescence microscopy (TIRFM), available ATP concentration is much lower than that in the in vivo environment. To achieve single-molecule observation with a high signal-to-noise ratio, zero-mode waveguides (ZMWs) are utilized even at high fluorescent molecule concentrations in the micromolar range.

Unconventional Universality Class of One-Dimensional Isolated Coarsening Dynamics in a Spinor Bose Gas.

By studying the coarsening dynamics of a one-dimensional spin-1 Bose-Hubbard model in a superfluid regime, we analytically find an unconventional universal dynamical scaling for the growth of the spin correlation length, which is characterized by the exponential integral unlike the conventional power law or simple logarithmic behavior, and numerically confirmed with the truncated Wigner approximation.

Dynamic formation of a microchannel array enabling kinesin-driven microtubule transport between separate compartments on a chip.

Microtubules driven by kinesin motors have been utilised as "molecular shuttles" in microfluidic environments with potential applications in autonomous nanoscale manipulations such as capturing, separating, and/or concentrating biomolecules. However, the conventional flow cell-based assay has difficulty in separating bound target molecules from free ones even with buffer flushing because molecular manipulations by molecular shuttles take place on a glass surface and molecular binding occurs stochastically; this makes it difficult to determine whether molecules are carried by molecular shuttles or by diffusion. To address this issue, we developed a microtubule-based transport system between two compartments connected by a single-micrometre-scale channel array that forms dynamically via pneumatic actuation of a polydimethylsiloxane membrane.

Colocalization of quantum dots by reactive molecules carried by motor proteins on polarized microtubule arrays.

The field of microfluidics has drastically contributed to downscale the size of benchtop experiments to the dimensions of a chip without compromising results. However, further miniaturization and the ability to directly manipulate individual molecules require a platform that permits organized molecular transport. The motor proteins and microtubules that carry out orderly intracellular transport are ideal for driving in vitro nanotransport.

Long-term observation of fluorescence of free single molecules to explore protein-folding energy landscapes.

A method was developed to detect fluorescence intensity signals from single molecules diffusing freely in a capillary cell. A unique optical system based on a spherical mirror was designed to enable quantitative detection of the fluorescence intensity. Furthermore, "flow-and-stop" control of the sample can extend the observation time of single molecules to several seconds, which is more than 1000 times longer than the observation time available using a typical confocal method.

Pressure effect on the electrical conductivity and superconductivity of beta-(BDA-TTP)2I3.

The pressure-induced electrical conductivity properties of beta-(BDA-TTP)2I3 have been investigated; the salt exhibits a dramatic change in the conductivity behaviour above ca. 10 kbar and undergoes a superconducting transition with an onset near 10 K.