tmRNA-dependent trans-translation is required for sporulation in Bacillus subtilis.

Spore formation in Bacillus subtilis is significantly impaired by the deletion of the gene for tmRNA (ssrA), which facilitates the trans-translation reaction that rescues stalled ribosomes and degrades incompletely synthesized peptides. Microscopic analysis revealed that the sporulation of most DeltassrA cells is blocked after forespore formation. Expression analysis of lacZ-fused genes directed by several RNA polymerase sigma factors showed that the synthesis of active sigma(K), encoded by the sigK gene, is predominantly inhibited in DeltassrA cells.