Novel purification method of human immunoglobulin by using a thermo-responsive protein A.

We attempted to evaluate a novel purification method of immunoglobulins (IgGs) by using a mutant type of protein A. Although this mutant protein A binds to IgGs at 5°C, the IgGs are released at 40°C; hence, it was designated as thermo-responsive protein A (TRPA). We aimed to purify IgG1 from the culture supernatant of CHO cells producing AE6F4 human monoclonal IgG1.

Evaluation of an anion-exchange hollow-fiber membrane adsorber containing γ-ray grafted glycidyl methacrylate chains.

It is widely recognized that membrane adsorbers are powerful tools for the purification of biopharmaceutical protein products and for this reason a novel hollow-fiber AEX type membrane adsorber has been developed. The membrane is characterized by grafted chains including DEA ligands affixed to the pore surfaces of the membrane. In order to estimate the membrane performance, (1) dynamic binding capacities for pure BSA and DNA over a range of solution conductivity and pH, (2) virus reduction by flow-through process, and (3) HCP and DNA removal from cell culture, are evaluated and compared with several other anion-exchange membranes.

[Experience in IVR performed using a posture support device designed especially for a patient unable to take the decubitus position].

We were requested to perform IVR for uterine artery embolotherapy of a patient who was unable to take the decubitus position. Because of extreme overweight, the patient was limited to a sitting posture on a bed in the living environment. Therefore, a device to support her sitting posture on the IVR patient table was designed and created.

[Newly developed monitor for IVR: liquid crystal display (LCD) replaced with cathode ray tube (CRT)].

For physicians who monitor images during interventional radiology (VR), we have built and been using a system that employs a liquid crystal display (LCD) instead of the conventional cathode ray tube (CRT). The system incorporates a ceiling-suspension-type monitor (three-display monitor) with an LCD on each of the three displays for the head and abdominal regions and another ceiling-suspension-type monitor (5-display monitor) with an LCD on each display for the cardiac region. As these monitors are made to be thin and light in weight, they can be placed in a high position in the room, thereby saving space and allowing for more effective use of space in the X-ray room.

[Clinical study with angiography system using a flat panel detecter].

We have been using an X-ray angiography system that incorporates a flat panel detector (FPD) since December 2001. This system is equipped with the scintillator-type FPD PaxScan 4030A from Varian Medical Systems, and for objective comparison of the image intensifier (I.I.

[An experience of the clinical study with angiography system using a Flat Panel Detector].

We had the chance of clinical studies by new proto-type Digital Subtraction Angiography (DSA) System with the Flat Panel Detector (FPD). That system has two types of detector, FPD and I.I.