Polyamidoamine dendron-bearing lipid assemblies: their morphologies and gene transfection ability.

Assembly morphology made from lipids is controlled by the balance between the polar headgroup and the hydrophobic tails. In this study, we showed the various generations of polyamidoamine dendron-bearing lipids could form various assembly morphologies. Furthermore, the effect of the assembly morphologies made from dendron-bearing lipids for transfection abilities were examined.

Development of an active targeting liposome encapsulated with high-density colloidal gold for transmission electron microscopy.

Active targeting of the liposome is an attractive strategy for drug delivery and in vivo bio-imaging. We previously reported the specific accumulation of Sialyl Lewis X (SLX) liposome to inflamed tissue in arthritic model mice or tumor-bearing mice. SLX-liposome encapsulation with fluorescent substances allows for the visualization of these liposomes by the time-dependent transvascular accumulation of fluorescent signals in the histological sections.

E-selectin targeting to visualize tumors in vivo.

Generally angiogenic factors induce the expression of E-selectin in vascular endothelial cells in the tumors. In this study, we employed an anti-E-selectin monoclonal antibody to target tumors in vivo and evaluated an optical imaging reagent to visualize tumor regions. The anti-E-selectin antibody was conjugated on the surface of liposomes, which encapsulated the near-infrared fluorescent substances Cy3 or Cy5.

Accumulation of liposome with Sialyl Lewis X to inflammation and tumor region: application to in vivo bio-imaging.

We prepared the liposome binding Sialyl Lewis X (SLX) on the surface in order to specifically and efficiently deliver substances (fluorescent materials, chemical substances, proteins, genes, etc.) to inflammation or tumor regions. The liposome with SLX (SLX-Lipo-Cy5.