Intracellular visualization of tetrodotoxin (TTX) in the skin of a puffer Tetraodon nigroviridis by immunoenzymatic technique.

Micro-distributions of tetrodotoxin (TTX) in the skin of a brackish-water puffer Tetraodon nigroviridis were investigated by means of a monoclonal anti-TTX antibody under light and transmission electron microscope. In light microscopy TTX antigen was visualized as brown color in undifferentiated basal cells and succiform cells of the skin, while in electron microscopy TTX was detected as black dots of immunogold in lysosomes of basal cells. From the results, it can be inferred that when TTX from the blood plasma of T.

Secretory glands of tetrodotoxin in the skin of the Japanese newt Cynops pyrrhogaster.

Intra-tissue distribution of tetrodotoxin (TTX) in the skin of larva, juvenile, and adult Japanese newt Cynops pyrrhogaster was investigated by means of a monoclonal antibody-based immunoenzymatic technique. In the investigation, TTX was localized at immature glands in juvenile, and at the granular cells composing of granular and mixed glands in adult specimens under a light microscope. No specific stain was recognized in larval section.