[Clinical-biochemical and immunological parameters in the diagnosis and treatment of chronic pyelonephritis on the background of intercurrent diseases].

Studies have shown that complicated chronic pyelonephritis in the active phase is characterized by structural and functional instability cytomembranes and decreased immunological resistance of the patient man. Supplement standard antibiotic treatment with ozone therapy antioxidant immunomodulation drug thus received immunobiochemical study.

[Clinicobiochemical and immunological assessment of polyoxidonium efficacy in the treatment of patients with chronic calculous pyelonephritis].

Of 59 patients with chronic calculous pyelonephritis (CCP) taking preparation therapy for extracorporeal lithotripsy, 29 patients received combined basic treatment (antibacterial drugs, phytotherapy, physiotherapy) plus polyoxidonium (a course of 10 intramuscular 6 mg injections each other day). The rest 30 patients (controls) received basic therapy alone. Polyoxidonium efficacy was assessed by the results of clinical, device and immunological investigations, content of the main phospholipid fractions and cholesterol in red cell membranes.

[Immunological indices in urolithiasis patients with secondary pyelonephritis].

The immunological status was studied in 335 patients with urolithiasis and pyelonephritis. The techniques used for assessment of the immunological status provided a multifactorial control over immune system function in inflammation and urolithiasis: registered a significant rise of the level of proinflammatory and anti-inflammatory mediators of the immune response (IL-1beta, IL-4, IL-6, TNF), of neutrophil metabolic activity with parallel suppression of neutrophil phagocytic and absorbing functions, inhibition of bactericidal activity of the serum, a significant fall in the number of mature T-lymphocytes (CD3+) and T-helpers (CD4+). The immune status indices in urolithiasis patients with secondary pyelonephritis are closely linked with clinico-laboratory manifestations of the inflammatory process and renal function.

[Endovascular Helium-Neon laser irradiation of the blood in the treatment of chronic pyelonephritis].

The authors analyze the condition of free-radical oxidation and activity of antioxidant system, clinical effectiveness of He-Ne laser therapy of patients with chronic pyelonephritis. It is shown that clinical manifestation of the disease is accompanied with activation of free radical oxidation and hypoactivity of antioxidant system. Endovascular laser hemotherapy stimulates antioxidant system, activity of superoxide dismutase, in particular.

[Polyoxidonium in the treatment of calculous pyelonephritis].

Efficacy of polyoxidonium was studied in combined therapy of urolithiasis complicated with secondary pyelonephritis. Of 60 patients with secondary pyelonephritis in urolithiasis, 30 received adjuvant polyoxidonium. Phospholipids and cholesterol in cytomembranes were defined with thin-layer chromatography.

[Lipids content in renal tissue membranes in patients with urolithiasis and secondary pyelonephritis].

We studied cytomembranes of the interstitial tissue of renal medullary layer obtained by lifetime biopsy of renal tissue in the course of pyelolithotomy in 46 patients with urolithiasis and secondary pyelonephritis. Biopsy structure was controlled with histological methods using standard staining. Basic phospholipids and cholesterol fractions in renal tissue cytomembranes were assayed at thin layer chromatography.

[Clinical and biochemical manifestations of chronic pyelonephritis and their correction in population of the northen areas of the Tyumen Territory].

Aim: To study clinicobiochemical features of chronic pyelonephritis (CPN) in the population of northen areas of the Tyumen Territory; to determine emoxipine effects on CPN treatment efficacy.

Material And Methods: Clinicolaboratory manifestations of CPN were studied in 246 CPN patients (126 residents of the Tyumen South and 120 residents of the Tyumen North, age 18-55, 174 females, 72 males). The control group consisted of 60 healthy subjects of the same gender and age.

[Homeostatic and antioxidant defense enzymes activity in chronic pyelonephritis].

As shown by the analysis of chronic pyelonephritis, manifestations in patients with high and low activity of antioxidant system enzymes, patients with high catalase activity have more effective defense of red cells, glomerular epithelium. This was concluded from marked differences in urinary excretion of metabolites, hemoglobin levels compared to those in patients with low activity of catalase. In low activity of glutathione peroxidase, inflammation gains activity, nonspecific resistance weakens, excretion of some metabolites inhibits vs patients with high activity of this enzyme.

[Membrane-destabilizing processes as the universal basis of inflammation].

It is established that active pyelonephritis and operative stress are accompanied with structural and functional changes in erythrocytic membranes: cholesterol and its ethers rose in quantity as well as difficulty oxidized phospholipids, cation adenosine triphosphatase activity changed. Being nonspecific, these changes seemed more pronounced in the active phase of chronic pyelonephritis. Minor structural rearrangement of membrane lipids in surgical patients indicated uneventful postoperative period.

[Autogenous infections in the development of postoperative complications].

The authors studied the mechanisms of bacterial dissemination in operative wounds in patients after various surgical interventions. It was demonstrated in experiments that during adaptation of the organism to the operative stress, autobacteria enter the blood from the intestinal reservoir and are eliminated through the urinary tract and wound surfaces, if foci of mechanical or toxic destruction exist the autobacteria precipitate in the affected tissues and participate directly in the reparative processes. On the basis of the obtained data it is suggested that the phenomenon of activation and persistence of endogenous microflora exists as one of the syndromes of adaptation of man to the aggression factor, which is aimed at revealing and removing the foci of tissue destruction developing as the result of exposure to the effect of the stress.

[Asymptomatic bacteriuria as the manifestation of a syndrome of the activation and persistence of endogenous microflora in dynamic human adaptation to stress].

The analysis has been performed of the mechanisms underlying the origin of asymptomatic bacteriuria as an adaptive reaction in response to surgical aggression. The experiments proved penetration of autobacteria from the intestine to blood channel and organic structures of the animals in the course of adaptation to an operative trauma and consequent elimination of the bacteria from the body. It was observed that endogenic microflora participates in regeneration of macroorganism structures which have lost their morphofunctional integrity due to mechanic and toxic aggression.

[The indices of cell membrane destabilization in urolithiasis patients].

The paper gives the results of a clinical and biochemical study of 165 patients with urolithiasis and 46 healthy persons. Their urinary samples and renal biopsy specimens were analysed to assess the structural and functional status of nephron cell membranes. In urolithiasis patients, the urine was found to contain a great quantities of emulsified lipids enriched in toxic phospholipid and cholesterol metabolism products; renal tissue cytomembranes in the patients were depleted of phospholipids, free cholesterol and enriched in phospholipid lysoforms and cholesterol esters.

[The enzyme function of cationic transmembrane transport and its relationship to the homeostatic indices of patients with chronic pyelonephritis].

The paper examines the relationship between the clinical manifestations of pyelonephritis and the functional activity of enzymes of cation transmembrane erythrocyte transport (Mg(2+)-, N(+)-K(+)-, Ca(2+)-ATPases). An individual analysis ascertained that the patients who showed a low Ca(2+)-ATPase activity had marked signs of inflammation in the body, as evidenced by ESR, seromucoid and fibrinogen concentrations. These patients had more significantly depressed immune defense mechanisms as reflected by the levels of immunoglobulins, T-lymphocytes, complement, the neutrophil phagocytosis, and urinary IgA concentrations).

[The treatment of coralliform nephrolithiasis].

A total of 184 patients with dendritic nephrolithiasis (74.5% females and 25.5% males) were under study.

[Urinary excretion of emulsified lipids in urolithiasis].

Examinations of the urine emulsified lipid phospholipid composition carried out by thin-layer chromatography have revealed that phospholipid excretion is the minimal in normal subjects and is represented only by sphingomyelin and phosphatidylcholine fractions. In subjects with a history of cholelithiasis (CL) phospholipid excretion is essentially elevated and the total phospholipid spectrum, including the minor fractions, is present in the urine. In CL patients phospholipiduria+ is still more marked, phosphatidylethanolamine appears in the urine, that is absent in the other groups of examinees.