Resolved genomes of wastewater ESBL-producing and metagenomic analysis of source wastewater samples.

Extended-spectrum beta-lactamase (ESBL)-producing pose a serious threat to human health because of their resistance to the most commonly prescribed antibiotics: penicillins and cephalosporins. In this study, we provide a genomic and metagenomic context for the determinant beta-lactam resistance genes of ESBL-positive isolated from various wastewater treatment utilities in Oregon, USA. Class A beta-lactamase genes on chromosomes (CTX-M, TEM) were clustered with antibiotic resistance genes associated with other classes of antibiotics (sulfonamides and aminoglycosides) along with insertional elements.