Detection of microcystin-producing cyanobacteria in water samples using loop-mediated isothermal amplification targeting gene.

Microcystin toxin-producing cyanobacteria are known to have harmful effects on humans and animals. We have developed a loop-mediated isothermal amplification (LAMP)-based detection method by targeting the microcystin synthetase B gene (), the gene responsible for the production of microcystin. The sensitivity of the method was found to be 1 fg per reaction, and it was 1000-fold higher than the conventional PCR.

Evaluation of analgesic and anti-inflammatory activity of a combination of tramadol-ibuprofen in experimental animals.

Background: Pain is the major concern of patients attending dental clinics, and satisfactory pain relief has always been difficult to achieve. Since the pathophysiology of pain is a complex, central and peripheral nervous system process, combined analgesic regimens with different mechanisms of action as a multimodal approach are becoming popular among the clinicians and dentists.

Objectives: The aim of the present study was to evaluate the analgesic and anti-inflammatory activity of ibuprofen and tramadol when used alone or in combination in animal models of pain and inflammation.

Study of Alzheimer’s Disease Progression In MR Brain Images based on Segmentation and Analysis of Ventricles using Modified DRLSE Method and Minkowski Functionals.

In this work, the ventricles in MR brain images are segmented using edge based modified Distance Regularized Level Set Evolution (DRLSE) method and the structural changes in the disease is further analysed using Minkowski functionals (MFs). Twenty normal and abnormal T1-weighted coronal mid slice MR image are considered for the analysis. The MR brain image is pre-processed using contrast enhancement method.

Crystal structure of (E)-1-([1,1'-biphen-yl]-4-yl)-3-(3-nitro-phen-yl)prop-2-en-1-one.

In the title compound, C21H15NO3, the mol-ecule has an E conformation about the C=C bond, and the C-C=C-C torsion angle is -178.24 (18)°. In the mol-ecule, the planes of the terminal rings are twisted by an angle of 42.

Crystal structure of (E)-1-(4'-methyl-[1,1'-biphen-yl]-4-yl)-3-(3-nitro-phen-yl)prop-2-en-1-one.

In the title compound, C22H17NO3, the mol-ecule has an E conformation about the C=C bond, and the C-C=C-C torsion angle is -177.7 (3)°. The planes of the terminal benzene rings are twisted by 41.

Crystal structure of (E)-1-(4'-meth-oxy-[1,1'-biphen-yl]-4-yl)-3-(3-nitro-phen-yl)prop-2-en-1-one.

The title compound, C22H17NO4, crystallizes with two independent mol-ecules (A and B) in the asymmetric unit. Each mol-ecule exists as an E isomer with C-C=C-C torsion angles of -175.69 (17) and -178.

Crystal structure of (Z)-3-{3-(4-chloro-phen-yl)-2-[(4-chloro-phen-yl)imino]-2,3-di-hydro-thia-zol-4-yl}-2H-chromen-2-one.

In the title compound, C24H14Cl2N2O2S, the 2H-chromene ring system is approximately planar, with a maximum deviation of 0.025 (2) Å. The thia-zole ring is almost planar, with an r.

3-Methyl-benzo[1,2-c:5,4-c']dichromen-6(8H)-one.

The title compound, C21H14O3, crystallizes with eight independent mol-ecules (A-H) in the asymmetric unit which are arranged in four groups of two mol-ecules each (AB, CD, EF and GH). In each mol-ecule, the pyran-2-one ring is planar (r.m.

(E)-1-([1,1'-Biphen-yl]-4-yl)-3-(2-methyl-phen-yl)prop-2-en-1-one.

In the title mol-ecule, C22H18O, the o-tolyl ring is connected through a conjugated double bond. The mol-ecule adopts an E conformation and the C-C=C-C torsion angle is 178.77 (13)°.

Di-aqua-tetra-kis-(1H-imidazole-κN (3))magnesium dichloride.

In the title compound, [Mg(C3H3N2)4(H2O)2]Cl2, the Mg(II) cation lies on a crystallographic inversion centre and is coordinated by two water mol-ecules and four N-atom donors from monodentate imidazole ligands, giving a slightly distorted octa-hedral stereochemistry. In the crystal, water O-H⋯Cl and imidazole N-H⋯Cl hydrogen bonds give rise to a three-dimensional structure.

rac-4a,10b-cis,10b,5c-trans-5-(7-Methyl-2-oxo-2H-chromen-4-yl)-3,4,4a,5,6,10b-hexa-hydro-2H-pyrano[3,2-c]quinoline.

In the racemic title compound, C(22)H(21)NO(3), the nitro-gen-containing ring of the pyran-oquinoline moiety adopts a slightly distorted half-chair conformation and the oxygen-containing ring adopts a slightly distorted chair conformation. The benzene rings make a dihedral angle of 84.97 (8)°.

3-Chloro-methyl-6,7-dimethyl-1,2-benz-oxazole.

In the title compound, C(10)H(10)ClNO, the benzoisoxazole ring is almost planar (r.m.s.

3-[Chloro-(phen-yl)meth-yl]-6-methyl-1,2-benzoxazole.

The title compound, C(15)H(12)ClNO, is a functionalized 1,2-benzoxazole with a chloro-(phen-yl)methyl substituent. The mol-ecule is V-shaped, the dihedral angle between the mean plane of the 1,2-benzoxazole system [maximum deviation = 0.023 (3) Å for the N atom] and the phenyl ring being 70.

Enhanced lipid peroxidation and impaired enzymic antioxidant activities in the erythrocytes of patients with cervical carcinoma.

This study examined the relationship between oxidative stress and enzymic antioxidant status in the erythrocytes of thirty-two adult cervical cancer patients and an equal number of age-matched cervicitis patients and healthy subjects. Lipid peroxidation was significantly increased, while the activities of enzymic antioxidants (superoxide dismutase, catalase, glutathione peroxidase) and glucose-6-phosphate dehydrogenase were decreased in the erythrocytes of cervical cancer patients as compared to healthy subjects and cervicitis patients. Thus, this study has demonstrated elevated lipid peroxidation and impaired enzymic antioxidant activities in the erythrocytes of cervical cancer patients.

Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients.

Background: Our aim was to examine the structural integrity of red blood cells in cervical cancer patients by measuring the concentrations of thiobarbituric acid reactive substances (TBARS), antioxidant status, cholesterol/phospholipid (C/P) molar ratio, enzyme activity and osmotic fragility of erythrocytes.

Methods: This study has been conducted on 32 adult female cervical cancer patients and an equal number of age- and sex-matched normal subjects. Erythrocyte concentrations of lipids, TBARS, vitamin E, reduced glutathione and enzymic activities of catalase and Na(+)K(+)-ATPase were measured as well as plasma concentrations of sodium and potassium.

Lipid peroxidation and antioxidant status in cervical cancer patients.

The present study examined the relationship between lipid peroxidation and antioxidant status in plasma, erythrocyte and erythrocyte membrane of cervical cancer patients and an equal number of age and sex matched normal subjects as well as patients with cervicitis. Lipid peroxidation was significantly increased with concomitant decrease in antioxidant levels in cervical cancer patients as compared to normal subjects and patients with cervicitis. The elevated lipid peroxidation and disturbed antioxidant status was also noticed in cervicitis patients as compared to normal subjects.