Relationships between the age of 25,445 men attending infertility clinics and sperm chromatin structure assay (SCSA®) defined sperm DNA and chromatin integrity.

Objective: To determine relationships between age of men with potential male factor infertility and sperm chromatin structure assay (SCSA) measures of sperm DNA fragmentation (SDF) and high DNA stainable sperm (HDS), and to compare these data with those obtained from healthy donor men without reproductive issues.

Design: Retrospective study.

Setting: Infertility clinics and diagnostic laboratory.

Luminal fluid of epididymis and vas deferens contributes to sperm chromatin fragmentation.

Study Question: Do the luminal fluids of the epididymis and the vas deferens contribute to sperm chromatin fragmentation (SCF) in mice?

Summary Answer: The luminal fluids of both organs are required for activating SCF in mice, but the vas deferens luminal fluid does this more efficiently than that of the epididymis.

What Is Known Already: Mice sperm have the ability to degrade their DNA in an apoptotic-like fashion when treated with divalent cations in a process termed SCF. SCF has two steps: the induction of reversible double-strand DNA breaks at the nuclear matrix attachment sites, followed by the irreversible degradation of DNA by nuclease.

Boar fertility and sperm chromatin structure status: a retrospective report.

Little information exists about boar sperm chromatin quality and fertility within a commercial setting. The objective of this report is to provide information about boar sperm chromatin integrity and its relationship to fertility. The sperm chromatin structure assay (SCSA) was used retrospectively to characterize sperm from 18 sexually mature boars having fertility information.

Analysis of sperm DNA fragmentation using flow cytometry and other techniques.

The goal of a rapid, precise and objective sperm measure of sperm DNA fragmentation, in situ, was realised a quarter century ago by the pioneer development of the sperm chromatin structure assay (SCSA). Since then, measurements on many thousands of sperm samples from a variety of animals and humans exposed to reproductive toxicants and having different diseases or infertility pathologies have solidly established the utility of sperm DNA fragmentation in experimental and clinical settings. New methods have been developed that measure sperm DNA fragmentation by different means such as the enzymatic labelling of broken DNA strands (Tunel assay by light microscopy or flow cytometry) and the light microscope observations of DNA fragments in the Comet assay and the negative loops of sperm chromatin in the Halo sperm assay.

Significant decrease in sperm deoxyribonucleic acid fragmentation after varicocelectomy.

Objective: To measure sperm DNA integrity values before and after varicocelectomy in patients with elevated preoperative levels of sperm DNA fragmentation.

Design: Retrospective.

Setting: Private urology clinic.

Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay.

Objective: To investigate how moderate and/or high levels of DNA fragmentation (DFI), as measured by the sperm chromatin structure assay (SCSA), affect either IVF or IVF with intracytoplasmic sperm injection (ICSI) fertilization, cleavage, blastulation, implantation, and pregnancy.

Design: Retrospective clinical study.

Setting: Academic human reproduction laboratory.

Chemopreventive effects of dietary mustard oil on colon tumor development.

Fatty acid composition of dietary fat is one of the detrimental factors in colon cancer development. Fats containing omega 6-polyunsaturated fatty acids (e.g.