Cloning vehicles for the homologous Bacillus subtilis host-vector system.

A series of Bacillus subtilis plasmids was constructed which carry either the leu region or both the leu and the dihydrofolate reductase (DHFR) regions of the B. subtilis chromosome. The DHFR-coding gene was derived from a trimethoprim resistant (Tmpr) B.

Cervical radiculomyelopathy caused by deposition of calcium pyrophosphate dihydrate crystals in the ligamenta flava. Case report.

A case of cervical radiculomyelopathy caused by multiple calcified nodules in the ligamenta flava is presented. Roentgenological examination of the cervical spine showed radiopaque nodular lesions, 7 x 7 x 5 mm in size, located in the paramedian portion of the posterior spinal canal. The nodules were removed surgically and they were confirmed to be calcifications of ligamenta flava.

Diffuse cerebrospinal gliomatosis. Case report.

A case of diffuse cerebrospinal gliomatosis is presented, with widespread involvement of the brain, cranial nerves, and spinal cord. This showed a far more extensive distribution of tumor cells than previously reported cases of gliomatosis cerebri. The clinical picture and oncogenesis of gliomatosis cerebri is briefly discussed.

Male prolactin secreting pituitary adenomas in humans studied by peroxidase-labelled antibody method.

In order to identity the source of excess secretion of prolactin in male patients, the pituitary tumors of 11 males with hyperprolactinaemia were studied by peroxidase-labelled antibody method using antibodies against human prolactin. Using light microscope, prolactin was demonstrated in most tumour cells in all cases. Ultrastructurally, prolactin was localized mainly on the secretory granules.

[Influence of the thickness of metal and porcelain upon metal-ceramics (author's transl)].

This paper deals with the strength of metal-ceramics and the influence of the thickness of metal and porcelain layer. The metal-ceramic specimens, 30 mm long and 10 mm wide of various each thickness, supported at each ends, were tested by bending to obtain the transverse strength. The thickness of metal layer was changed from 0.

Spontaneous transdural extension of malignant astrocytoma. Case report.

A case of malignant astrocytoma in the frontoparietal parasagittal region with transgression into the overlying dura mater and the skull is presented. Spontaneous transdural extension of a glioma is an extremely rare growth pattern. A mode of transdural extension of this tumor is discussed and related reports are reviewed.

Histological observation of the brain of Tay-Sachs disease with seizure and chronic DPH intoxication--report of an autopsy case.

The patients was a 3-year-old boy with psychomotor retardation and attacked by seizures since 8 months of age. On funduscopy, the maculla presented a cherry-red spot. Serum hexosaminidase A activities were as low as 8.

Hepatic dearterialization in 3'-methyl-4-dimethylaminoazo-benzene-induced hepatocellular carcinoma with special reference to circulatory dynamics and mitochondrial functions.

This time trend of hemodynamics and mitochondrial functions were studied to determine whether the ligation of the hepatic artery would result in an antitumor effect on 3'-methyl-4-dimethylaminoazobenzene-induced hepatic carcinoma in rats. The studies revealed that the hepatic tumors were nourished predominantly by the artery and less by the portal vein; the size of the vascular beds in the hepatic tumors decreased as compared with those in the non-tumor area; and as the tumors grew larger, the artery became less predominant and the size of vascular beds decreased further. The mitochondria in the tumor were characterized by impaired growth, impaired oxidative phosphorylation, and by the low activity and nucleotide specificity of membrane bound ATPase.