Facemask and Rapid Maxillary Expansion With Alternative Rapid Maxillary Expansion and Constriction Protocol in the Management of Skeletal Class III Malocclusion.

The correction of skeletal class III malocclusions is one of the most difficult orthodontic treatments. Skeletal Class III malocclusion may result from a combination of maxillary deficits and mandibular prognathism, mandibular prognathism alone, or maxillary deficits alone. Treatment options include an orthopedic appliance (facemask and chin cup), orthodontics with camouflage, a combination of orthognathic surgery and orthodontics, and the recently introduced bone-anchored maxillary protraction.

Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures.

Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-induced cytotoxicity are studied.

3D cell culture systems: advantages and applications.

Cell cultures are important material of study for the variety of advantages that they offer. Both established continuous cell lines and primary cell cultures continue to be invaluable for basic research and for direct applications. Technological advancements are necessary to address emerging complex challenges and the way cells are cultured in vitro is an area of intense activity.

Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity.

Biosynthesis of silver nanoparticles (AgNPs) was achieved by a novel, simple green chemistry procedure using citrus sinensis peel extract as a reducing and a capping agent. The effect of temperature on the synthesis of silver nanoparticles was carried out at room temperature (25°C) and 60°C. The successful formation of silver nanoparticles has been confirmed by UV-vis, FTIR, XRD, EDAX, FESEM and TEM analysis and their antibacterial activity against Escherichia coli, Pseudomonas aeruginosa (gram-negative), and Staphylococcus aureus (gram-positive) has been studied.