The discovery of novel noncoding RNAs in 50 bacterial genomes.

Structured noncoding RNAs (ncRNAs) contribute to many important cellular processes involving chemical catalysis, molecular recognition and gene regulation. Few ncRNA classes are broadly distributed among organisms from all three domains of life, but the list of rarer classes that exhibit surprisingly diverse functions is growing. We previously developed a computational pipeline that enables the near-comprehensive identification of structured ncRNAs expressed from individual bacterial genomes.

8-oxoguanine riboswitches in bacteria detect and respond to oxidative DNA damage.

Riboswitches rely on structured aptamer domains to selectively sense their target ligands and regulate gene expression. However, some riboswitch aptamers in bacteria carry mutations in their otherwise strictly conserved binding pockets that change ligand specificities. The aptamer domain of a riboswitch class originally found to selectively sense guanine forms a three-stem junction that has since been observed to exploit numerous alterations in its ligand-binding pocket.

A conserved uORF in the mRNA of species regulates operon expression.

Computational methods can be used to identify putative structured noncoding RNAs (ncRNAs) in bacteria, which can then be validated using various biochemical and genetic approaches. In a search for ncRNAs in , we observed a conserved region called the -II motif located upstream of the gene that is also present in other members of this genus. This gene codes for an enzyme involved in the production of branched-chain amino acids (BCAAs).

Task Sharing for Managing Common Noncommunicable Disease in a Nurse Led Noncommunicable Diseases Clinic in Peri-Urban Community of Chandigarh.

Background: Nurse led noncommunicable diseases (NCD) clinic may address the significant shortage of human resource for health for managing common NCDs. The objective of this study is to assess the feasibility and effectiveness of nurse-led NCD clinic for identification, prevention, and management of common NCDs.

Materials And Methods: A quasi experimental study was conducted at a Public Health Dispensary in periurban community of Northern India.

Discovering riboswitches: the past and the future.

Riboswitches are structured noncoding RNA domains used by many bacteria to monitor the concentrations of target ligands and regulate gene expression accordingly. In the past 20 years over 55 distinct classes of natural riboswitches have been discovered that selectively sense small molecules or elemental ions, and thousands more are predicted to exist. Evidence suggests that some riboswitches might be direct descendants of the RNA-based sensors and switches that were likely present in ancient organisms before the evolutionary emergence of proteins.

Nurse-led cardiovascular diseases risk assessment and communication using WHO/ISH risk prediction chart in a tertiary care hospital of North India.

Cardiovascular diseases (CVDs) contribute to most of the potentially preventable burden through early risk assessment. Nurse-led CVD risk assessment is an effective strategy to address the human resource crisis for CVD prevention. An interventional study was conducted in medicine wards of a tertiary care hospital in North India to train nurses in CVD risk assessment and its communication.

An study to Compare Dental Laser with other Treatment Modalities on Biofilm Ablation from Implant and Tooth Surfaces.

Background: Periodontal and peri-implant disorders are etiologically linked to bacterial biofilms. The researchers wanted to see how well the erbium-doped yttrium aluminum garnet (Er:YAG) laser removed bacterial biofilms along with attached epithelial cells (EC), gingival fibroblasts (GF), in addition to osteoblast-like cells (OC) dentin along with titanium surfaces compared to previous therapy methods.

Methodology: 3.

In vitro Evaluation of Laser vs. Handpiece for Tooth Preparation.

Background: The erbium-doped yttrium aluminium garnet (Er:YAG) laser has been revealed to effectively ablate dental hard tissues, and its utilisation to caries eradication and cavity preparation is envisaged. Nevertheless, only a limited research has been performed on the Er:YAG laser's capacity to treat caries.

Aim And Objectives: The efficiency of caries elimination with an Er:YAG laser was equated to that of traditional mechanical therapy in this study.

Multiple in vivo roles for the C-terminal domain of the RNA chaperone Hfq.

Hfq, a bacterial RNA chaperone, stabilizes small regulatory RNAs (sRNAs) and facilitates sRNA base-pairing with target mRNAs. Hfq has a conserved N-terminal domain and a poorly conserved disordered C-terminal domain (CTD). In a transcriptome-wide examination of the effects of a chromosomal CTD deletion (Hfq1-65), the Escherichia coli mutant was most defective for the accumulation of sRNAs that bind the proximal and distal faces of Hfq (Class II sRNAs), but other sRNAs also were affected.

Assessment of Oral Health Status with Visually Impaired Children in Patna City, Bihar.

Aim: Children with a visually impairment often face various challenges in their day-to-day skills. Oral hygiene maintenance is one major task for them. Such child has difficulty to live current normal life and demands of permission or healthy life.

Assessment of Effect of Different Sterilization Agents on Dimensional Accuracy of Different Impression Materials in Implant Prosthesis - An Study.

Aim: Sterilization of impression materials is of paramount importance. The present study was conducted to compare the effect of different disinfectants on dimensional accuracy of elastomeric impression materials used for implant prosthesis and other routine treatments.

Materials And Methods: The present study was conducted with polyvinyl siloxane (PVS) (regular body), PVS (medium body), PVS (heavy body), and polyether (medium body) impression materials.

Resistance against Fracture in Teeth Managed by Root Canal Treatment on Restoring with Onlays, Inlays, and Endocrowns: A Comparative Analysis.

Aim And Objective: To compare the fracture resistance in teeth managed by root canal treatment after restoring with different types of onlays, inlays, and endocrowns prepared with hybrid ceramics and pulp chambers restored with fiber-reinforced composite and resin composite that were radiopaque, light-cured, and flowable.

Materials And Methods: The present study was carried out on 252 extracted mandibular molars. All the specimens were divided into six groups randomly.

Predictors and prevalence of periodontitis among pregnant women of slum areas of Patna, India: An opportunity for oral health promotion.

Background: There is abundant documentation in literature that presence of maternal periodontal infections has been attributed to serious health problem to the mother and the child. Regular evaluation of any illness burden is required for planning preventive and treatment strategies and fills the existing health gap. There is a lack of literature about the predictors and prevalence of periodontal diseases in expectant women in the slum areas of Patna.

Need of Palliative Care Services in Rural Area of Northern India.

Background: Palliative care is comprehensive care that provides symptomatic relief and enhances the quality of life for people experiencing serious health-related suffering. There is an increasing need for palliative care services in India. Estimates for population requiring these services are essential in order to meet the increasing need for palliative care services.

Assessment of Efficacy of 5% Topical Amlexanox and 0.1% Topical Triamcinolone Acetonide in Management of Recurrent Aphthous Stomatitis.

Aim: The aim of this study was to assess the efficacy of 5% topical amlexanox and 0.1% topical triamcinolone acetonide in recurrent aphthous stomatitis (RAS) management.

Materials And Methods: Sixty adult patients of RAS of both genders were divided into two groups with each group having 30 patients.

Assessment of Aluminum Chloride Retraction Cords, Expasyl, and Tetrahydrozoline-Soaked Retraction Systems in Gingival Retraction.

Aim: The aim of this study was to assess different gingival displacement systems such as aluminum chloride retraction cords, expasyl, and tetrahydrozoline-soaked retraction cord to record intracrevicular margins of tooth preparations.

Materials And Methods: This study included 60 patients. Patients were divided into four groups of 15 each.

To Compare Mineral Trioxide Aggregate, Platelet-rich Fibrin, and Calcium Hydroxide in Teeth with Irreversible Pulpitis: A Clinical Study.

Aim: Numerous pulp-capping agents such as mineral trioxide aggregate (MTA), calcium hydroxide (Ca(OH)), platelet-rich fibrin (PRF), and biodentine have been suggested. This clinical study was conducted to compare MTA, PRF, and Ca(OH) in teeth with irreversible pulpitis.

Materials And Methods: The 60 mandibular molar teeth with irreversible pulpitis were used in this study, which were restored with Ca(OH), MTA, and PRF.

Familial oral hygiene practices and its influence among rural youths-exploring primary preventive measures.

Background And Aims: A child usually takes up the behavioural habits by observing the parents at home. The mapping of the current level of influence of parents' practices over children is necessary for developing any preventive programs therefore the aim of the study was to determine the influence of familial oral health habits on the adolescent children belonging to rural background.

Method: The present cross-sectional questionnaire study was conducted on 236 rural participants (parents and their children) reporting to a private dental college in a rural set up in India.

Factors affecting patient satisfaction among patients undergone removable prosthodontic rehabilitation.

Background: Patient's satisfaction is of utmost importance to the dental professionals among all those who have undergone prosthodontic rehabilitation. The success of every dental prosthesis lies in the patient wearing those removable prostheses thereby restoring masticatory function and Phonatics, hence the aim of the present study is to determine various factors affecting the patient satisfaction among patients undergone removable Prosthodontic rehabilitation.

Materials And Methods: The present study is a cross-sectional questionnaire descriptive study.

Correlation between lip print patterns and skeletal class I and II malocclusions - A tool to diagnose early.

Background: Cheiloscopic analysis has been shown to have close association with skeletal malocclusion. Hence, aim of the present study was to explore any association between lip prints patterns and skeletal class I and II malocclusions.

Materials And Methods: A study sample of 90 subjects aged between 18 and 25 years was selected from individuals opting for orthodontic therapy.

The prevalence of molar incisor hypomineralization of school children in and around Muvattupuzha, Kerala.

Background: Molar incisor hypomineralization (MIH) is a developmental dental defect and has a significant impact on the quality of life of affected individuals. Most of the prevalence studies of MIH have been carried out in the European countries; very little data are available from India.

Aim: The aim and objective of this study was to determine the prevalence of MIH in 8-15-year-old Malayalee school children in and around Muvattupuzha, Kerala.

New aspects of RNA-based regulation by Hfq and its partner sRNAs.

Hfq, an RNA chaperone, promotes the pairing of small RNAs (sRNAs) to target mRNAs, mediating post-transcriptional regulation of mRNA stability and translation. This regulation contributes to bacterial adaptation during stress and pathogenesis. Recent advances in sequencing techniques demonstrate the presence of sRNAs encoded not only in intergenic regions but also from the 3' and 5' UTRs of mRNAs, expanding sRNA regulatory networks.

C-terminal domain of the RNA chaperone Hfq drives sRNA competition and release of target RNA.

The bacterial Sm protein and RNA chaperone Hfq stabilizes small noncoding RNAs (sRNAs) and facilitates their annealing to mRNA targets involved in stress tolerance and virulence. Although an arginine patch on the Sm core is needed for Hfq's RNA chaperone activity, the function of Hfq's intrinsically disordered C-terminal domain (CTD) has remained unclear. Here, we use stopped flow spectroscopy to show that the CTD of Escherichia coli Hfq is not needed to accelerate RNA base pairing but is required for the release of dsRNA.