Raman spectroscopy of intact feline corneal collagen.

The Raman Spectrum of Collagen is presented from feline corneas which were fresh and intact, heat denatured, and incubated in 2H20. Two bands in the amide I region at approx. 1630 cm-1 and approx.

Pyridinium- and quinolinium-2-dithioacetic acid zwitterions: antiradiation and anticancer activities.

Pyridinium-, quinolinium-, and one pyrimidinium-2-dithioacetic acid zwitterions were prepared by condensation of the N-methyl heterocyclic anhydro bases with carbon disulfide. Reaction of the 2-methylpyridine methiodide anhydro base with carbon disulfide resulted in replacement of the 2-methyl group to give the 1-methylpyridinium-2-dithioacetic acid zwitterion. The 1,6-dimethylquinolinium-2-dithioacetic acid zwitterion showed appreciable anticancer activity against P-388 lymphocytic leukemia in mice, but the other zwitterions tested showed no activity.

The noneffect of a large linear hydrocarbon, squalene, on the phosphatidylcholine packing structure.

The interaction of squalene with liposomes and monolayers of dipalmitoyl phosphatidylcholine (DPL) has been studied by differential scanning calorimetry, Raman spectroscopy, and surface potential measurements. Mole ratios of squalene to DPL up to 9 to 1 were studied. In contrast to small, nonpolar molecules, which profoundly influence the structure of lipid bilayers as detected by changes in both their thermodynamic phase transition parameters and membrane fluidity, this large, nonpolar, linear hydrocarbon is devoid of such influences.

Being imitated by an adult and the subsequent imitative behavior of retarded children.

Institutionalized retarded subjects (N=57) were assigned to one of three experimental conditions during a simple three-choice task in which the subjects and the experimenter took turns feeding colored plastic "Cookie Monster." In the imitation condition, the experimenter imitated the subject's choice, and in the ninimtation condition, the experimenter avoided imitating the subject. Following imitation or nonimitation, the subject was given opportunities to imitate the experimenter.

Experimental pneumonia due to Pseudomonas aeruginosa in leukopenic dogs: prolongation of survival by combined treatment with passive antibody to Pseudomonas and granulocyte transfusions.

Treatment with type-specific IgG antibody to Pseudomonas aeruginosa significantly increased rates of survival after experimental induction of pseudomonas pneumonia in leukopenic dogs. Longer survival times were correlated with higher titers of circulating antibody in serum; however, no animals treated with antibody alone were long-term survivors. Subsequent development of sepsis or the recovery of Pseudomonas from infected lung tissue was not altered by treatment with antibody.

Interactions between segmented polyurethane surfaces and the plasma protein fibrinogen.

Surfaces of a segmented polyurethane were varied by casting on poly(ethylene terephthalate) (PET) and glass substrates, and were characterized through infrared-attenuated total-reflection spectroscopy (ATR). Surfaces cast on glass substrates showed a higher content of polyether segments, whereas those cast on PET contained a higher relative concentration of aromatic segments. Adsorption, and possible conformational changes of fibrinogen, were found to be more substantial on polymer surfaces having a higher content of polyether segments.

Capacitance studied of syntheric phospholipid Langmuir films.

Synthetic phosphatidylcholine Langmuir films have been incorporated into metal-insulator-metal (MIM) thin film junctions. The capacitance characteristics of these junctions have been studied as a function of temperature, the number of lipid layers in the insulating layer, and the length of the hydrocarbon chains of the lipid molecule. The thickness of the oxide layer on the base aluminum electrode has been determined to be larger than or equal to 11 A, and its effects on the capacitance characteristics have been considered in some detail.

Laser Raman spectroscopy of lipid-protein systems. Differences in the effect of intrinsic and extrinsic proteins on the phosphatidylcholine Raman spectrum.

Laser Raman spectroscopy is used to examine the interactions of intrinsic and extrinsic proteins with the lipid layer structure. The interactions of cytochrome c and cytochrome c oxidase with lipids have been well established by others using a variety of techniques. Cytochrome c is thought to act as an extrinsic membrane protein while cytochrome c oxidase is thought to act as an intrinsic membrane protein.

Raman spectroscopic detection and examination of the interaction of amino acids, polypeptides and proteins with the phophatidylcholine lamellar structure.

Raman spectral peaks in the vicinity of 1100 and 2900 cm-1 for phosphatidylcholine were found to be sensitive to interactions with amino acids, polypeptides and plasma proteins. The amino acids L-luecine, L-isoleucine, L-tryptophan, L-arginine HCl, L-histidine HCl, L-threonine and L-aspartic acid decreased the dipalmitoyl phosphatidylcholine Raman intensity ratio I1064/I1089 indicating an increase in the gauche hydrocarbon chain character of the lipid. The increase in the lipid approx.

Relationship between cognitive tempo and selective attention in learning disabled children.

The relationship between cognitive tempo and selective attention in 29 learning disabled children was investigated. The performance of the 9 reflective and the 9 impulsive children on selective-attention tasks was compared. An examination of t tests and correlations between variables for each task showed a relationship between cognitive tempo and selective attention.

Effect of ions on phospholipid layer structure as indicated by Raman spectroscopy.

Various anions and cations are found to induce changes in the layered structure of phosphatidylcholine-water systems as indicated by Raman Spectroscopy. From the ratio of Raman intensities, I1064/I1089, it is inferred that dipositive ions decrease the proportion of gauche character in the hydrocarbon chains, with the relative influence being: Ba2+ less than Mg2+ less than Ca2+ similar to Cd2+. Unipositive ions (Li+, K+ and Na+) produce no observed changes in the Raman spectrum of the lecithin dispersion.

Antiradiation compounds XV: condensations of carbon disulfide with amino, chloro, cyanomethyl, and sulfonamido heterocycles.

Condensations of carbon disulfide were carried out with amino, chloro, and diamino heterocycles to give condensed ring thiazoline-2-thiones and imidazoline-2-thiones, with cyanomethyl heterocycles to give dithio acid derivatives, and with heterocyclic sulfonamides to give sulfonyldithiocarbamates. Of several examples tested, pyrido[3,2-d]thiazoline-2-thione, disodium 2-(5-chloro-2-thienyl)-3,3-dimercaptoacrylonitrile, triethylammonium 4-sulfamoylphenyldithiocarbamate, ammonium beta-phenethyldithiocarbamate, and methyl N-(thiophene-2-sulfonyl)dithiocarbamate, only the last-named compound showed any radiation protection for mice. Several compounds gave negative tests for antimalarial activity.

Parents' predictions of their children's perceptions of family relations.

Thirty children from 15 families were administered the Bene-Anthony Family Relations Test. Their parents then assigned test responses as they imagined their children had. With only two exceptions, both involving first born boys, parents predicted their children's responses beyond chance expectation.

A calorimetric and monolayer investigation of the influence of ions on the thermodynamic properties of phosphatidylcholine.

The effects of various ions and 2H2O on the thermal properties of phosphatidylcholine dispersions were studied using differential scanning calorimetry and the change in the surface potential of monolayers with temperature. The phosphatidylcholine in 2H2O dispersion exhibits a slightly higher transition temperature and lower enthalpy of melting than a phosphatidylcholine in H2O dispersion. Monovalent (H+, Na+, and Li+) and some divalent cations of chloride salts (Ba2+, Mg2+, and Sr2+) have no effect on the thermal properties of phosphatidylcholine, while halide salts of the di-positive ions Cd2+ and Ca2+ have an effect on both the enthalpy of melting and transition temperature.