Isotope ratio monitoring by C NMR spectrometry (irm-C NMR) provides the complete C intramolecular position-specific composition at natural abundance. It represents a powerful tool to track the (bio)chemical pathway which has led to the synthesis of targeted molecules, since it allows Position-specific Isotope Analysis (PSIA). Due to the very small composition range (which represents the range of variation of the isotopic composition of a given nuclei) of C natural abundance values (50‰), irm-C NMR requires a 1‰ accuracy and thus highly quantitative analysis by C NMR.
View Article and Find Full Text PDF