Publications by authors named "Katushi Yamaguchi"
Article Synopsis
- The CRISPR/Cas9 system is a key tool for genome editing, crucial for producing gene-edited plants, particularly T0-generation trees that are analyzed for mutations.
- T0-generation plants often display unexpected mutations, making it important to identify those with desirable mutation patterns through analyzing both alleles and ensuring the absence of somatic chimerism.
- This study employed fragment analysis and Oxford Nanopore Technologies (ONT) amplicon sequencing to effectively assess and confirm mutation patterns and chimerism in gene-edited Cryptomeria japonica, highlighting ONT's cost-effectiveness for plant genetic research.
Background: In ipecac (Carapichea ipecacuanha (Brot.) L. Andersson), adventitious shoots can be induced simply by placing internodal segments on phytohormone-free culture medium.
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