Bioassay-guided isolation and in Silico characterization of cytotoxic compounds from Hemimycale sp. Sponge targeting A549 lung cancer cells.

Bioassay-guided fractionation approach led to identification of two novel compounds; (4-(hydroxymethyl)-3-methoxy-1H-pyrazol (1) and mycalene (2), alongside with four known metabolites; octadecane (3), hexatriacontane (4), 1-heneicosanol (5) and heptatriacontanoic acid (6) from the Red Sea marine sponge Hemimycale sp. The ethyl acetate fraction showed a noticeable cytotoxic activity against the lung cancer cell line (A549) with IC value of 75.54 µg/ mL.

New sesquiterpenes from the soft coral Litophyton arboreum.

Two new sesquiterpenes; 8α,11-dihydroxy-β-cyperon (2), and 5-epi-7α-hydroxy-( +)-oplopanone (3), were isolated from the soft coral Litophyton arboreum, together with nine known ones, including five sesquiterpenes; 11-hydroxy-8-oxo-β-cyperon (1), alismoxide (4), 5β,8β-epidioxy-11-hydroxy-6-eudesmene (5), chabrolidione B (6), 7-oxo-tri-nor-eudesm-5-en-4β-ol (7), two sterols; 7β-acetoxy-24-methyl-cholesta-5,24(28)-diene-3β,19-diol (8), nebrosteroid M (9), and two glycerol derivatives; chimyl alcohol (10) and batyl alcohol (11). The structures of the isolated compounds were characterized using spectroscopic techniques, predominately HR-ESI-MS, 1D, 2D-NMR, and ECD analyses. Compounds 1-11 were evaluated for their cytotoxic activity against three human cancer-cell lines (A549, MCF-7 and HepG2), and anti-leishmanial potential against the causal parasite, Leishmania major.

Comparative Study of Liposomal and Ethosomal Formulations of Curcuma heyneana Rhizome Extract in a Transdermal Delivery System.

Aim: This study aimed to develop an anti-aging nanoformulation with Curcuma heyneana extract as bioactive substance.

Background: Curcuma heyneana Valeton & Zipj extract has been proven in previous research to have antioxidant, anti-ageing, anti-inflammatory, and wound healing properties, which makes it a potential bioactive material for anti-ageing and sunscreen cosmetic products. Phytoantioxidants need to penetrate into deeper skin layers to ensure effectivity.

Anti-leishmanial and cytotoxic compounds isolated from marine sponge .

The methanolic extract of the marine sponge . yielded two new compounds; 1-(2'-methyl heptadecyl) phenol () and a new pyrazole derivative; 4-(hydroxymethyl)-1-pyrazol-3-ol (), together with previously isolated (2')-2'-hydroxy-N-((2,3,4)-1,3,4-trihydroxy-16-methylpentadecan-2-yl)docosanamide (), cholesterol (), 5, 8-dioxycholest-6-en-3-ol () and 3-acetylsesterstatin 3 (), which were firstly reported from family Hymedesmiidae. Their structure elucidation was based on extensive nuclear magnetic resonance spectroscopy and high resolution-electrospray ionization-mass spectrometry.

Effects of (mill.) urb. bulb extract on mice glucocorticoid-induced osteoporosis models.

Background: Low bone mass accompanied by microarchitectural alterations in the bone that cause fragility fractures is known as secondary osteoporosis and occurs when there is an underlying condition or medication present. Eleutherine bulbosa bulb extract has been shown to affect bone because of its content, which can help osteoblast differentiation and inhibit osteoclast differentiation.

Objective: This study aimed to assess the effects of 70% ethanol extract of Bulbs (EBE) from Pasuruan-East Java on blood calcium levels, osteoblast cell count, and bone density of trabecular femur in osteoporosis rats.

study of phytochemicals contained in Brucea javanica in inhibiting the InhA enzyme as antituberculosis.

Background: Currently is found to be resistant to the treatment of tuberculosis with rifampin and isoniazid (INH) and often stated as multi-drug resistance (MDR). Knowledge and determination of biological properties of plant extracts is a source of drug candidates in various health fields. Therefore, natural products are important in the discovery of new drugs, especially in disease therapy, particularly for tropical diseases, tuberculosis.

Antiosteoarthritis activities of 70% ethanol extract of (mill.) urb. bulb on rats monosodium iodoacetate-induced osteoarthritis.

Background: Osteoarthritis (OA) is a common degenerative joint situation that induces pain and disability in the elderly. Traditionally, bulb from Pasuruan, East Java, is used to treat many diseases, also as an anti-inflammatory.

Objective: In this research, we employed an model to examine the effects of 70% ethanol extracts of (EBE) on the progression and development of OA.

Two New Cytotoxic Sesquiterpene-Amino Acid Conjugates and a Coumarin-Glucoside from .

The Asteraceae family is a promising source of bioactive compounds, such as the famous Asteraceae plants (pyrethrin) and (artemisinin). As a result of our series of phytochemical studies of the subtropical plants, two novel sesquiterpenes, named crossoseamines A and B in this study ( and , respectively), one undescribed coumarin-glucoside (), and eighteen known compounds (-) were isolated from the aerial part of (Asteraceae). The structures of isolated compounds were elucidated by spectroscopic methods, including 1D and 2D NMR experiments (H, C, DEPT, COSY, HSQC, HMBC, and NOESY), IR spectrum, circular dichroism spectrum (CD), and high-resolution electrospray ionization-mass spectrometry (HR-ESI-MS).

Immunomodulatory effect from ethanol extract and ethyl acetate fraction of Valeton and Zijp: Transient receptor vanilloid protein approach.

This study aims to discover the immunomodulatory potential of the ethanol extract (EE) and the ethyl acetate fraction (EAF) of Valeton and Zijp (Indonesian name: temu giring) rhizome using mice models. The affinity of the curcuminoid (curcumin, dimethoxy-, and bisdemethoxy-) through the Transient Receptor Potential Vanilloid 1 (TRPV1) was determined using Mollegro molecular docking in silico. The curcuminoid concentration of the EE and EAF of rhizome were determined using thin-layer chromatography densitometry.

Synthesis, In Silico Study, Antibacterial and Antifungal Activities of -phenylbenzamides.

Antibiotic and antifungal resistance problems have been prevalent in recent decades. One of the efforts to solve the problems is to develop new medicines with more potent antibacterial and antifungal activity. -phenylbenzamides have the potential to be developed as antibacterial and antifungal medicine.

Human Lung Cancer (A549) Cell Line Cytotoxicity and Anti- Activity of Leaves: A Study Supported by UPLC-ESI-MS/MS Metabolites Profiling and Molecular Docking.

Lung cancer and cutaneous leishmaniasis are critical diseases with a relatively higher incidence in developing countries. In this research, the activity of leaf hydromethanolic extract and its solvent-fractions (-hexane, EtOAc, -butanol, and MeOH) against the lung adenocarcinoma cell line (A549) and was investigated. The MeOH fraction exhibited higher cytotoxic activity (IC 1.

Complete Genome Sequence of sp. Strain L3-i23 Isolated from Soil in Japan.

This study describes the complete genome sequence of sp. strain L3-i23, acquired from an assembly of long reads and subsequently polished using short reads. The complete genome comprises a 3,139,863-bp chromosome with a GC content of 69.

L. var. Leaf Extract Alleviates Gentamicin-Induced Hepatorenal Injury in Rats-Possible Modulation of IL-1β and NF-κB Activity Assisted with Computational Approach.

Background: Recently, crop byproducts are considered a hot topic and can be converted into beneficial products. Cauliflower is well-known for its protective effects against oxidative stress-induced damage. The current study aimed to investigate the chemical profile and the ameliorative effects of cauliflower leaf extract (CL) on gentamicin-induced renal and hepatic injuries in rats.

Aureanin: a new iridoid from the leaves of (Silva Manso) Benth. & Hook.f. ex S.Moore.

One new iridoid named aureanin () was isolated from the leaves of (Silva Manso) Benth. & Hook.f.

Four new glucosides from the aerial parts of Equisetum sylvaticum.

Two previously undescribed megastigmane glucosides, (3S)-3-hydroxy-4-oxo-7,8-dihydro-β-ionone-3-O-β-D-glucopyranoside (1), (3S)-3-hydroxy-4-oxo-β-ionone-3-O-β-D-glucopyranoside (2), an apocarotenoid glucoside named equiseoside A (3) and an unusual aromatic compound with a glucose-fused skeleton named equiseoside B (4), together with 35 known compounds (5-39) were isolated from the aerial parts of Equisetum sylvaticum. The structures of these compounds were elucidated by spectroscopic methods, including 1D and 2D NMR, IR, CD, and HR-MS.

Isolation and structural characterization of phytoconstituents from leaves of .

Phytochemical investigation of leaves led to the isolation of three new compounds: including a glycoside of simple alcohol, namely binatoside (), 3,4-dihydroxy--methyl piperidin-2-one (), and a phenyl ethanoid glycoside, namely bignanoside C (), alongside with five known compounds; including a glycoside of simple alcohol; (2) propane-1,2-diol 1--(6--caffeoy1)--D-glucopyranoside (), phenyl ethanoids; leucosceptoside A () and plantainoside C (), and iridoids; ipolamiide () and strictoloside (). The structure of the isolated compounds was elucidated by various spectroscopic methods, including 1 D and 2 D NMR experiments, HR-ESI-MS as well as by comparison with the literature.

Draft Genome Sequence of sp. Strain L3-i20, Isolated from Soil in Japan.

This study describes the draft genome sequence of sp. strain L3-i20, obtained from an assembly of long reads and subsequently polished using short reads. The draft genome comprises a 5,308,756-bp chromosome with a GC content of 41.

Ameliorative Effect of Benth on Diabetic, Apoptotic, and Adipogenic Biomarkers of Diabetic Rats and 3T3-L1 Fibroblasts Assisted by In Silico Approach.

Diabetes mellitus (DM) is a complicated condition that is accompanied by a plethora of metabolic symptoms, including disturbed serum glucose and lipid profiles. Several herbs are reputed as traditional medicine to improve DM. The current study was designed to explore the chemical composition and possible ameliorative effects of on blood glucose and lipid profile in high-fat diet/streptozotocin-induced diabetic rats and in 3T3-L1 cell lines as a first report of its bioactivity.

Aspects of the Complement System in New Era of Xenotransplantation.

After producing triple (Gal, H-D and Sd)-KO pigs, hyperacute rejection appeared to no longer be a problem. However, the origin of xeno-rejection continues to be a controversial topic, including small amounts of antibodies and subsequent activation of the graft endothelium, the complement recognition system and the coagulation systems. The complement is activated via the classical pathway by non-Gal/H-D/Sda antigens and by ischemia-reperfusion injury (IRI), via the alternative pathway, especially on islets, and via the lectin pathway.

Cytotoxic and antileishmanial triterpenes of (Silva Manso) leaves.

The bioactivity-guided fractionation of the total ethanolic extract of the leaves of revealed the cytotoxic and antileishmanial potency of the ethyl acetate fraction, in which its phytochemical investigation resulted in the isolation of five triterpenes; identified as oleanolic acid (), ursolic acid (), pomolic acid (), tormentic acid (), 3,6,19-trihydroxy-urs-12-en-28-oic acid () in addition to one triterpenoid glucoside, spathodic acid 28---D-glucopyranoside (). Whereas compound showed cytotoxic activity against three different cell lines; A549, MCF-7 and HepG2 with IC values of 31.7 ± 1.

Crotofolane-Type Diterpenoids: Crotocascarins R-V, Rearranged Trinorcrotofolane: Crotocascarin δ and a Phorbol Derivative from the Leaves of Croton cascarilloides.

Five new crotofolanes, named crotocascarins R-V (1-5), one rearranged trinorcrotofolane, crotocascarin δ, and one phorbol derivative were isolated from the EtOAc-soluble fraction of the MeOH extract of the leaves of Croton cascarilloides. Crotocascarins R (1), T (3), and U (4) possessed isobutyric acid as an acyl moiety and crotocascarin B (2) an acetyl group, whereas crotocascarin V (5) was elucidated to be a hydroxylated compound of crotocascarin K at the 9-position. Crotocascarin δ (6) was a trinor rearranged crotofolane with a tertiary hemiketal functional group at the 8-position.

Effect of a C5a receptor antagonist on macrophage function in an intestinal transplant rat model.

Background: C5a promotes alloreactivity via the C5a receptor 1 (C5aR1) on immune cells, but this has not been confirmed in the case of small intestine transplantation immunity. In the present study, we examined the effect of C5aR1 antagonist (PMX53) on macrophage function in small intestinal transplantation.

Methods: The model was created by heterotopic intestinal transplantation using donor Dark Agouti and recipient Lewis rats.