Surfactant degradation activity in bronchoalveolar lavage fluid from guinea pigs challenged with antigen.

Background And Objective: Surfactant dysfunction is a characteristic of bronchial asthma, but mechanisms of dysfunction following antigen exposure are not understood. The aim of this study was to examine whether bronchoalveolar lavage fluid (BALF) has surfactant degradation activity after antigen challenge, using an animal model of asthma.

Methods: BALF was collected 24 h after a challenge with aerosolized antigen solution in actively sensitized guinea pigs and from non-sensitized control guinea pigs.

Effects of surfactant replacement on irregular overdistension of meconium-injured lungs in rats.

Background: Overdistension of the lungs is a cause of ventilator-induced lung injury. In meconium aspiration syndrome, irregular overdistension of the lungs often occurs.

Objectives: We investigated whether surfactant replacement could restore the terminal airspaces in the lungs that had been distended after meconium aspiration.

[Anesthetic management for awake laparoscopic surgery for ectopic pregnancy in a patient with heterotopic pregnancy].

A 26-year-old woman with heterotopic pregnancy in the 8th week of gestation was scheduled for surgery for ectopic pregnancy. To reduce the effect on the intrauterine pregnancy, awake laparoscopic surgery was performed under spinal anesthesia with pneumoperitoneum pressure of 6 mmHg. Bilateral shoulder pain occurred when pneumoperitoneum pressure was increased to 8 mmHg.

Lung volumes and alveolar expansion pattern in immature rabbits treated with serum-diluted surfactant.

In acute respiratory distress syndrome, mechanical ventilation often induces alveolar overdistension aggravating the primary insult. To examine the mechanism of overdistension, surfactant-deficient immature rabbits were anesthetized with pentobarbital sodium, and their lungs were treated with serum-diluted modified natural surfactant (porcine lung extract; 2 mg/ml, 10 ml/kg). By mechanical ventilation with a peak inspiration pressure of 22.

Modified protocols for surfactant therapy in experimental meconium aspiration syndrome.

In adult rats with experimental meconium aspiration syndrome, we investigated whether the therapeutic effect of exogenous surfactant was increased by addition of dextran or preceding airway lavage with diluted surfactant. Animals (n = 72) ventilated with pure oxygen were given human meconium suspension (50-75 mg kg(-1)) through the airways. When the PaO(2) had decreased to <20 kPa (mean +/- SD 12 +/- 3.

Effects of various forms of surfactant protein C on tidal volume in ventilated immature newborn rabbits.

Surfactant protein (SP)-C is characterized by alpha-helix structure and palmitoyl groups attached to two cysteine residues. We examined the function of palmitoylation and dimerization in promotion of tidal volume in immature newborn rabbits. Reconstituted surfactants were made from a mixture of synthetic phospholipids and porcine SP-B (basic mixture) by adding various forms of SP-Cs: normal SP-C isolated from porcine lungs and monomeric or dimeric forms of SP-C.