The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factors.

Unlabelled: In bacteria, if a ribosome translates an mRNA lacking a stop codon it becomes stalled at the 3' end of the message. These ribosomes must be rescued by -translation or the alternative rescue factors (ArfA or ArfB). However, mounting evidence suggests that the ribosome quality control (RQC) pathway may also rescue non-stop ribosomes.

Evaluation of Surrogate Tests for the Presence of -Mediated Methicillin Resistance in Staphylococcus capitis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus warneri.

Testing of staphylococci other than (SOSA) for -mediated resistance is challenging. Isolates of , , , and were evaluated by cefoxitin and oxacillin broth microdilution (BMD), disk diffusion (DD), and PBP2a immunoassay, and the results were compared to PCR results. No phenotypic susceptibility test correlated well with PCR results across all species, although the PBP2a immunoassay yielded 100% correlation.

Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as the cause of a worldwide pandemic. Many commercial SARS-CoV-2 reverse transcription-PCR (RT-PCR) assays have received Emergency Use Authorization from the U.S.

Evaluation of Oxacillin and Cefoxitin Disk Diffusion and Microbroth Dilution Methods for Detecting -Mediated β-Lactam Resistance in Contemporary Staphylococcus epidermidis Isolates.

Methicillin (β-lactam) resistance in is mediated by the gene, with resistance reported to be as high as 90%. The goal of this study was to evaluate oxacillin and cefoxitin disk diffusion (DD) and broth microdilution (BMD) methods for the detection of -mediated β-lactam resistance in 100 human isolates of (48 -positive isolates and 52 negative isolates). Oxacillin DD tests using the Clinical and Laboratory Standards Institute (CLSI) M100-S28 breakpoints for / accurately differentiated -positive and -negative isolates, with categorical agreement (CA) of 100% and no very major errors (VMEs) or major errors (MEs) identified.

Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini.

The group (SIG) is a collection of coagulase-positive staphylococci consisting of four distinct species, namely, , , , and SIG members are animal pathogens and rare causes of human infection. Accurate identification of has important implications for interpretation of antimicrobial susceptibility testing data and may be important for other members of the group. Therefore, we sought to evaluate the performance of five commercially available identification platforms with 21 isolates obtained from a variety of animal and geographic sources.

Activity of Imipenem-Relebactam and Comparator Agents against Genetically Characterized Isolates of Carbapenem-Resistant Enterobacteriaceae.

Carbapenem-resistant (CRE) strains are an urgent public health threat. We evaluated the activities of 19 antimicrobial agents, including imipenem-relebactam, against (i) 106 CRE bloodstream isolates that primarily expressed carbapenemase (KPC) and (ii) 20 OXA-48-like-expressing CRE isolates. Ninety-five percent of CRE bloodstream isolates were susceptible to imipenem-relebactam.