Chemosensory Ca2+ dynamics correlate with diverse behavioral phenotypes in human sperm.

In the female reproductive tract, mammalian sperm undergo a regulated sequence of prefusion changes that "prime" sperm for fertilization. Among the least understood of these complex processes are the molecular mechanisms that underlie sperm guidance by environmental chemical cues. A "hard-wired" Ca(2+) signaling strategy that orchestrates specific motility patterns according to given functional requirements is an emerging concept for regulation of sperm swimming behavior.

Odorant receptors and olfactory-like signaling mechanisms in mammalian sperm.

Since their discovery in 1991, members of the odorant receptor (OR) family have been found in various ectopic tissues, including testis and sperm. It took, however, more than a decade for the first mammalian testicular ORs to be functionally characterized and implicated in a reproductively relevant scenario. Activation of hOR17-4 and mOR23 in human and mouse sperm, respectively, mediates distinct flagellar motion patterns and chemotactic behavior in various bioassays.

Particulate adenylate cyclase plays a key role in human sperm olfactory receptor-mediated chemotaxis.

Human sperm chemotaxis is a critical component of the fertilization process, but the molecular basis for this behavior remains unclear. Recent evidence shows that chemotactic responses depend on activation of the sperm olfactory receptor, hOR17-4. Certain floral scents, including bourgeonal, activate hOR17-4, trigger pronounced Ca(2+) fluxes, and evoke chemotaxis.