Publications by authors named "Katja KoSSmann"
We report on the rational engineering of the binding interface of the self-ligating HaloTag protein to generate an optimized linker for DNA nanostructures. Five amino acids positioned around the active-site entry channel for the chlorohexyl ligand (CH) of the HaloTag protein were exchanged for positively charged lysine amino acids to produce the HOB (halo-based oligonucleotide binder) protein. HOB was genetically fused with the enzyme cytochrome P450 BM3, as well as with BMR, the separated reductase domain of BM3.
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- Warfarin inhibits the VKORC1 enzyme, crucial for blood coagulation, but historical studies have shown widely varying results due to different assay conditions used for measuring its effects.
- The researchers standardized a DTT-driven VKOR activity assay to establish clearer methodologies for measuring enzymatic constants and warfarin's inhibitory concentration (IC50).
- They concluded that the Ki value (a more accurate measure of inhibition) is not directly equal to the IC50 value from DTT assays, suggesting that previous IC50 results may need revisiting to ensure accuracy and comparability across studies.*