Publications by authors named "Katja Fjeldstad"
Article Synopsis
- Madin-Darby canine kidney (MDCK) cells show resistance to the classical effects of brefeldin A (BFA), which typically affects transport in the Golgi apparatus.
- BFA reduces the sulfation of important molecules like heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) by inhibiting the uptake of a specific compound, adenosine 3'-phosphate 5'-phosphosulfate (PAPS), into the Golgi without altering its structure.
- The impact of BFA differs from that of chlorate, as lower concentrations of chlorate affect sulfation of CSPGs more than HSPGs;
Brefeldin A (BFA) perturbs the organization of the Golgi apparatus, such that Golgi stack components are fused with the endoplasmic reticulum (ER) and separated from the trans-Golgi network. In many cell types, BFA blocks the secretion of macromolecules but still allows the action of Golgi enzymes in the ER. Treatment of cells with BFA has been reported to inhibit the secretion of heparan sulphate (HS) proteoglycans and alter the structure of their HS components, but the nature of such structural alterations has not been characterized in detail.
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