Regulation by hypoxia of adrenomedullin output and expression in human trophoblast cells.

Objectives: Plasma adrenomedullin concentrations are increased in the fetal circulation in acute and chronic hypoxic conditions. The effect of hypoxia in regulating adrenomedullin synthesis and secretion was investigated in human placental trophoblast cells.

Study Design: Human trophoblast cells obtained from term placentas (n = 7) were cultured in hypoxic condition (3% oxygen).

Effect of corticosteroids and progesterone on adrenomedullin output and expression in human fetal membranes and placenta trophoblasts.

Objective: Plasma adrenomedullin (AM) concentrations are increased in fetal and maternal circulation in response to exogenous glucocorticoids administration. The role of corticosteroids and progesterone in regulating AM synthesis and secretion was investigated in amnion and chorion trophoblast cells of the fetal membranes and in placental trophoblast cells.

Study Design: Cells were treated with betamethasone, hydrocortisone, and progesterone.