Comparative performance of human papillomavirus messenger RNA versus DNA screening tests at baseline and 48 months in the HPV FOCAL trial.

Background: HPV FOCAL is a randomized trial comparing high-risk HPV [Hybrid Capture 2 (HC2)] vs. liquid-based cytology (LBC) for primary cervical screening.

Objective: The present study objective was to compare Aptima HPV (AHPV) and HC2 assay performance at the intervention arm baseline and 48 mo.

Post-Loop Electrosurgical Excision Procedure High-Risk Human Papillomavirus Testing as a Test of Cure: The British Columbia Experience.

Objectives: To determine whether Hybrid Capture 2 High-Risk HPV DNA Test (HC2) can be used as a test of cure in women treated for cervical intraepithelial neoplasia grade 2 or worse (CIN 2+) and allow discharge from colposcopy follow-up with a return to a cytology-based screening program for HC2-negative women.

Materials And Methods: Data were analyzed for all women who underwent a loop electrosurgical excision procedure between August 1, 2008, and June 30, 2011, and had a valid HC2 result after loop electrosurgical excision procedure and follow-up histopathology result, to determine risk of persistent or recurrent CIN 2+ in HC2-positive and HC2-negative women.

Results: Two thousand three hundred forty women had adequate biopsies and valid HC2 results.

Interobserver agreement for assessing invasion in stage 1A vulvar squamous cell carcinoma.

Invasive squamous cell carcinoma of the vulva with ≤1 mm stromal invasion is classified as stage 1A. Cancer staging systems state that the depth of invasion should be measured from the epithelial-stromal junction of the adjacent most superficial dermal papilla to the deepest point of the invasive tumor. Measurement of the depth of invasion guides patient management.

Reproducibility of the histological diagnosis of cervical dysplasia among pathologists from 4 continents.

The reliable histological diagnosis of cervical squamous intraepithelial lesions (SILs), especially low-grade SIL, is known to be problematic. Poor diagnostic reproducibility can complicate studies addressing its appropriate management. As part of an international study comparing expectant management of histologically proven low-grade SIL with immediate loop electrocautery excisional procedure, this study was carried out to assess interobserver agreement on the histological diagnosis of SILs among a group of 22 pathologists from 5 countries and the intraobserver reliability among a subset of 7 Canadian pathologists.