Production and purification of the penicillin-binding protein 3 from Pseudomonas aeruginosa.

The penicillin-binding proteins (PBPs) are peripheral membrane enzymes that catalyze the final steps for the biosynthesis of the essential bacterial cell wall heteropolymer peptidoglycan. Bacteria produce a number and variety of PBPs which are classified as either high molecular weight or low molecular weight PBPs. The high molecular weight PBPs are multimodular being comprised of an N-terminal membrane anchor followed by a non-penicillin binding domain and a C-terminal penicillin-binding domain.

Function of penicillin-binding protein 2 in viability and morphology of Pseudomonas aeruginosa.

Objectives: To investigate the function of penicillin-binding protein 2 (PBP 2) in Pseudomonas aeruginosa PAO1.

Methods: The growth and morphology of P. aeruginosa cultured in the absence and presence of mecillinam was assessed.