Publications by authors named "Kathryn P Sherry"
Article Synopsis
- The Notch signaling pathway is crucial for determining cell fate and is influenced by the ubiquitin ligase Deltex.
- Using NMR spectroscopy, researchers identified how Deltex interacts with the Notch ankyrin (ANK) domain, highlighting the N-terminal WWE motif as the binding site.
- Mutations in either Deltex or ANK disrupt their interaction and the enhancement of Notch transcriptional activation, while an unexpected finding reveals that Deltex can still bind to the Notch intracellular domain without its WWE domain, indicating a potential secondary interaction.
Intrinsically disordered regions (IDRs) play important roles in proteins that regulate gene expression. A prominent example is the intracellular domain of the Notch receptor (NICD), which regulates the transcription of Notch-responsive genes. The NICD sequence includes an intrinsically disordered RAM region and a conserved ankyrin (ANK) domain.
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- The interaction between the Notch intracellular domain (NICD) and the transcription factor CSL is crucial for turning on Notch-responsive genes.
- The RBP-j-associated molecule (RAM) region of NICD disrupts corepressor binding and helps anchor NICD to CSL, enhancing transcriptional activation through a process that resembles a worm-like chain model.
- Investigations into the RAM's structure showed it consists of transient helices and dynamic regions, with specific primary sequences influencing activation, rather than the secondary structures themselves.