Capillary electrophoretic assay of acyl-CoA hydrolase activity.

Acyl-CoA hydrolases catalyze the hydrolysis of fatty acyl CoA thioesters to free fatty acids and coenzyme A. These enzymes play an important role in the maintenance of cellular acyl-CoA and free CoASH pools and in the detoxification of nonphysiological metabolites. The assays most commonly used for acyl-CoA hydrolase quantitation are spectrophotometric and radioisotopic methods, both of which have limitations.